Muhandiram Subhashini, Karunarathna Thusitha K, Siriweera Eranga H, Ratnayake Chathura J, Kodithuwakku Suranga P
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Peradeniya, Peradeniya, 20400 Sri Lanka.
Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, 20400 Sri Lanka.
Virusdisease. 2024 Jun;35(2):271-280. doi: 10.1007/s13337-024-00875-w. Epub 2024 Jun 18.
High oncogenic risk types of human papillomaviruses are mainly transmitted via sexual contact and are the main cause of cervical cancer in females in developing countries. Molecular detection of HPV infection enables early cancer detection; however, it is not widely used in low-income countries due to resource constraints. The aim of this study was to assess economical yet sensitive HPV detection and genotyping assays for both physician and self-collected cervical samples in a resource limited diagnostic setting. A previously reported polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) based HPV detection and genotyping protocol was verified using direct DNA sequencing to accurately identify the HPV 16 and 18 genotypes in a routine-diagnostic set-up. Then the HPV prevalence in a cohort of 433 clinically normal females was performed using PCR-RFLP diagnostic tool. Finally, the performance of the PCR-RFLP HPV screening tool was further evaluated against self-collected samples. HPV 16 and 18 genotyping with the PCR-RFLP consistently agreed with the sequencing data. The HPV prevalence in the screening cohort was 5.8%. HPV 16 and 18 were the most common high-risk HPV genotypes detected in the study cohort. Self-sampling physician collected samples from the same subject resulted in an overall concordance of 93% for HPV detection. The PCR-RFLP protocol can be used effectively under low resource settings for HPV 16/18 diagnosis and genotyping. The self-sampling approach can be recommended to increase HPV screening among women in Sri Lanka.
The online version contains supplementary material available at 10.1007/s13337-024-00875-w.
高危型人乳头瘤病毒主要通过性接触传播，是发展中国家女性宫颈癌的主要病因。人乳头瘤病毒感染的分子检测能够实现癌症的早期发现；然而，由于资源限制，在低收入国家尚未广泛应用。本研究旨在评估在资源有限的诊断环境中，针对医生采集和自我采集的宫颈样本，经济且灵敏的人乳头瘤病毒检测及基因分型检测方法。在常规诊断环境中，使用直接DNA测序对先前报道的基于聚合酶链反应（PCR）和限制性片段长度多态性（RFLP）的人乳头瘤病毒检测及基因分型方案进行验证，以准确鉴定人乳头瘤病毒16型和18型。然后，使用PCR-RFLP诊断工具对433名临床正常女性队列中的人乳头瘤病毒感染率进行检测。最后，针对自我采集的样本，进一步评估PCR-RFLP人乳头瘤病毒筛查工具的性能。PCR-RFLP法进行的人乳头瘤病毒16型和18型基因分型结果与测序数据一致。筛查队列中人乳头瘤病毒感染率为5.8%。人乳头瘤病毒16型和18型是研究队列中检测到的最常见的高危型人乳头瘤病毒基因型。对同一受试者自我采样与医生采集的样本进行人乳头瘤病毒检测，总体一致性为93%。PCR-RFLP方案可在资源匮乏的环境中有效用于人乳头瘤病毒16/18型的诊断和基因分型。建议采用自我采样方法以提高斯里兰卡女性的人乳头瘤病毒筛查率。
网络版包含可在10.1007/s13337-024-00875-w获取的补充材料。
