Malik Tasbiha, Fatima Batool, Hussain Dilshad, Jabeen Fahmida, Jawad Shan E Zahra, Mohyuddin Abrar, Najam-Ul-Haq Muhammad
Department of Biochemistry, Bahauddin Zakariya University, Multan 60800, Pakistan.
HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
ACS Omega. 2024 Jul 9;9(29):31335-31343. doi: 10.1021/acsomega.3c08610. eCollection 2024 Jul 23.
ATP upregulation is a significant driver of aggressive cancer cell phenotypes. Phosphometabolites participate in metabolic pathways and are overexpressed in cancer cell activity. Therefore, developing novel and accurate methods for detecting phosphometabolites in biological fluids is essential. In this research, a novel zeolite composite comprising magnesium, aluminum, and lanthanum hydroxides (MALZ) is developed and used for the first time to enrich phosphorylated metabolites via its inherent interaction with phosphate groups. SEM micrographs show a crystalline cubic structure with a small diameter of 36.62 nm. FTIR analysis confirms the phosphate adsorption and desorption using AMP and ATP as the standards. XRD analysis of MALZ provides structural information about the synthesized composite. Adsorption-desorption parameters, such as pH, shaking time, and MALZ concentration, are optimized to analyze the binding capacity of the fabricated material for phosphorylated metabolites. A kinetic study reveals the rapid and effective AMP and ATP adsorptions on MALZ. The multiple hydroxyl groups of ternary hydroxides and high affinity of lanthanum toward the phosphate group enrich 26 phosphometabolites from serum samples of malignant neoplastic patients. The LC-MS profile shows characteristic phosphometabolites that may act as signatures of cancer-related abnormal metabolic pathways. This study may provide an experimental pathway for detecting metabolites in human body fluids.
ATP上调是侵袭性癌细胞表型的一个重要驱动因素。磷酸代谢物参与代谢途径，并在癌细胞活动中过表达。因此，开发用于检测生物体液中磷酸代谢物的新颖且准确的方法至关重要。在本研究中，首次开发并使用了一种包含氢氧化镁、氢氧化铝和氢氧化镧的新型沸石复合材料（MALZ），通过其与磷酸基团的固有相互作用来富集磷酸化代谢物。扫描电子显微镜图像显示出直径为36.62 nm的小尺寸立方晶体结构。傅里叶变换红外光谱分析以AMP和ATP作为标准物证实了磷酸盐的吸附和解吸。MALZ的X射线衍射分析提供了有关合成复合材料的结构信息。优化了诸如pH值、振荡时间和MALZ浓度等吸附 - 解吸参数，以分析所制备材料对磷酸化代谢物的结合能力。动力学研究揭示了MALZ对AMP和ATP的快速有效吸附。三元氢氧化物的多个羟基以及镧对磷酸基团的高亲和力从恶性肿瘤患者的血清样本中富集了26种磷酸代谢物。液相色谱 - 质谱图显示出可能作为癌症相关异常代谢途径特征的磷酸代谢物。本研究可能为检测人体体液中的代谢物提供一条实验途径。
