Shi Hui-Qin, Huang Shu, Ma Xin-Yue, Tan Zhen-Ju, Luo Rui, Luo Bei, Zhang Wei, Shi Lei, Zhong Xiao-Lin, Lü Mu-Han, Chen Xia, Tang Xiao-Wei
Department of Gastroenterology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China.
Department of Gastroenterology, The People's Hospital of Lianshui, Huaian 223499, Jiangsu Province, China.
World J Gastrointest Oncol. 2024 Jul 15;16(7):3082-3096. doi: 10.4251/wjgo.v16.i7.3082.
Hepatocellular carcinoma (HCC) is a malignant tumor that has a high incidence and mortality worldwide. Despite extensive studies, the detailed molecular mechanism of HCC development remains unclear. Studies have shown that the occurrence and development of HCC are closely related to abnormal gene expression. has been shown to be overexpressed in a variety of malignant tumors. However, the role of in HCC remains unclear.
To investigate the expression of and -related competing endogenous RNAs (ceRNAs) in HCC and their clinical significance, in order to provide new ideas for the diagnosis and treatment of HCC.
The data of HCC were obtained from the Cancer Genome Atlas database and The Genotype Tissue Expression, including transcriptome data and clinical information. Multiple common databases, including UALCAN, Timer 2.0, cBioPortal, LinkedOmics, starBase, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes, were used to analyse the expression of , prognostic value, genetic alteration, co-expressed genes, differentially expressed genes, gene-related ceRNAs and functional enrichment analysis in HCC patients. Kaplan-Meier analysis, univariate and multivariate Cox regression analysis were used to analyze survival prognosis and the Spearman test was used to measure correlations between and immune functions. And R language package was used to analyze the correlation between and immune invasion of HCC.
Our study indicated that was differentially expressed in various tumor tissues. The over-expression of gene was an unfavorable prognostic indicator for HCC patients, and associated with unfavorable cytogenetic risk and gene mutations. Moreover, most immune cells were positively correlated with ( < 0.05). According to the results of functional enrichment analysis, was involved in the positive regulation of epidermal growth factor receptor signaling pathway and ERBB signaling pathway, and was related to DNA replication and GTPase regulator activity. Finally, our study found that based on and pathways, targeting might promote the occurrence and development of HCC.
Collectively, this study indicated that the gene was involved in the occurrence and development of HCC, and it might be a new biomarker and therapeutic target for HCC, but the specific mechanism remains to be further verified.
肝细胞癌（HCC）是一种在全球范围内发病率和死亡率都很高的恶性肿瘤。尽管进行了广泛的研究，但HCC发生发展的详细分子机制仍不清楚。研究表明，HCC的发生发展与基因表达异常密切相关。 已被证明在多种恶性肿瘤中过表达。然而，其在HCC中的作用仍不清楚。
研究 及相关竞争性内源性RNA（ceRNA）在HCC中的表达及其临床意义，为HCC的诊断和治疗提供新思路。
从癌症基因组图谱数据库和基因型组织表达数据库获取HCC数据，包括转录组数据和临床信息。使用多个常用数据库，包括UALCAN、Timer 2.0、cBioPortal、LinkedOmics、starBase、基因本体论和京都基因与基因组百科全书，分析HCC患者中 的表达、预后价值、基因改变、共表达基因、差异表达基因、 基因相关ceRNA和功能富集分析。采用Kaplan-Meier分析、单因素和多因素Cox回归分析生存预后，采用Spearman检验测量 与免疫功能之间的相关性。并使用R语言包分析 与HCC免疫浸润之间的相关性。
我们的研究表明， 在各种肿瘤组织中差异表达。 基因的过表达是HCC患者不良预后的指标，且与不良的细胞遗传学风险和基因突变相关。此外，大多数免疫细胞与 呈正相关（ < 0.05）。根据功能富集分析结果， 参与表皮生长因子受体信号通路和ERBB信号通路的正调控，且与DNA复制和GTPase调节活性相关。最后，我们的研究发现，基于 和 通路，靶向 可能促进HCC的发生发展。
总体而言，本研究表明 基因参与了HCC的发生发展，它可能是HCC的一个新的生物标志物和治疗靶点，但具体机制仍有待进一步验证。
