CD25 和 CTLA4 基因转录水平在急性移植物抗宿主病早期预测中的应用。
Application of CD25 and CTLA4 gene transcription levels in early prediction of acute graft-versus-host disease.
机构信息
Department of Pediatrics, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
Department of Pediatrics, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, China.
出版信息
Front Immunol. 2024 Jul 12;15:1410439. doi: 10.3389/fimmu.2024.1410439. eCollection 2024.
INTRODUCTION
Our study investigated the potential of peripheral blood T cell CD25, CD28, and CTLA-4 gene transcription levels as predictive biomarkers for acute graft-versus-host disease (aGVHD) following allogeneic hematopoietic stem cell transplantation (allo-HSCT).
METHODS
Real-time reverse transcription fluorescent quantitative PCR (RT-qPCR) analysis was conducted on day +7, +14, and +21 post-transplantation in patients undergoing allo-HSCT.
RESULTS
Elevated levels of CD25 and CTLA-4 mRNA were found to be associated with the occurrence of aGVHD, as well as severe and gastrointestinal aGVHD. Receiver operating characteristic (ROC) curve analysis was utilized to assess the predictive value of each biomarker. Combined analysis of CD25 and CTLA-4 mRNA levels demonstrated promising predictive potential for aGVHD.
CONCLUSION
Our results confirmed that the transcription levels of CD25 and CTLA-4 genes could be used as early predictive biomarkers for aGVHD post-allo-HSCT.
简介
我们的研究调查了外周血 T 细胞 CD25、CD28 和 CTLA-4 基因转录水平作为异基因造血干细胞移植（allo-HSCT）后急性移植物抗宿主病（aGVHD）的预测生物标志物的潜力。
方法
对接受 allo-HSCT 的患者在移植后第+7、+14 和+21 天进行实时逆转录荧光定量 PCR（RT-qPCR）分析。
结果
发现 CD25 和 CTLA-4 mRNA 水平升高与 aGVHD 的发生以及严重和胃肠道 aGVHD 相关。利用受试者工作特征（ROC）曲线分析评估每个生物标志物的预测价值。CD25 和 CTLA-4 mRNA 水平的联合分析显示出对 aGVHD 有很好的预测潜力。
结论
我们的结果证实 CD25 和 CTLA-4 基因的转录水平可作为 allo-HSCT 后 aGVHD 的早期预测生物标志物。
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