多组分胺生物缀合用气体试剂。
Gaseous Reagent for Multicomponent Amine Bioconjugation.
机构信息
Department of Chemistry, Rice University, Houston, Texas 77005, United States.
Carbon Dioxide Activation Center (CADIAC), Interdisciplinary Nanoscience Center, Department of Chemistry, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.
出版信息
Org Lett. 2024 Aug 9;26(31):6608-6613. doi: 10.1021/acs.orglett.4c02246. Epub 2024 Jul 29.
We report a minimalist gaseous sulfonyl-chloride-derived reagent for multicomponent bioconjugation with amine, phenol, or aniline reagents to afford urea or carbamate products. With the utilization of a gas-phase reagent for a reaction mediated by metal ions, a variety of biologically relevant molecules, such as saccharide, poly(ethylene glycol), fluorophore, and affinity tag, can be efficiently cross-linked to the N terminus or lysine side-chain amines on natural polypeptides or proteins.
我们报道了一种极简的气态磺酰氯衍生试剂，可与胺、酚或苯胺试剂进行多组分生物偶联，生成脲或氨基甲酸酯产物。通过利用气相试剂进行金属离子介导的反应，可以有效地将各种生物相关分子，如糖、聚乙二醇、荧光团和亲和标签，交联到天然多肽或蛋白质的 N 末端或赖氨酸侧链氨基上。
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