Department of Life and Environmental Sciences, Laboratory of Fungal Interaction and Molecular Biology (Donated by IFO), University of Tsukuba, Ibaraki, Japan.
Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, Japan.
mSphere. 2024 Aug 28;9(8):e0034524. doi: 10.1128/msphere.00345-24. Epub 2024 Jul 29.
is a species in the order Mucorales that is known to cause mucormycosis, but it is poorly understood as a host of viruses. Here, we examined 25 clinical strains of for viral infection with a conventional double-stranded RNA (dsRNA) assay using agarose gel electrophoresis (AGE) and the recently established fragmented and primer-ligated dsRNA sequencing (FLDS) protocol. By AGE, five virus-infected strains were detected. Then, full-length genomic sequences of 12 novel RNA viruses were revealed by FLDS, which were related to the families and ill-defined groups of single-stranded RNA (ssRNA) viruses with similarity to the established families and and the proposed family "Ambiguiviridae." All the characterized viruses, except a potential phasmavirid with a negative-sense RNA genome, had positive-sense RNA genomes. One virus belonged to a previously established species within the family , whereas the other 11 viruses represented new species or even new genera. These results show that the fungal pathogen harbors diverse RNA viruses and extend our understanding of the diversity of RNA viruses in the fungal order Mucorales, division Mucoromycota. Identifying RNA viruses from clinical isolates of may expand the repertoire of natural therapeutic agents for mucormycosis in the future.IMPORTANCEThe diversity of mycoviruses in fungal hosts in the division Mucoromycota has been underestimated, mainly within the species . Only five positive-sense RNA genomes had previously been discovered in this species. Because current sequencing methods poorly complete the termini of genomes, we used fragmented and primer-ligated double-stranded RNA sequencing to acquire the full-length genomes. Eleven novel mycoviruses were detected in this study, including the first negative-sense RNA genome reported in . Our findings extend the understanding of the viral diversity in clinical strains of Mucoromycota, may provide insights into the pathogenesis and ecology of this fungus, and may offer therapeutic options.
是被囊动物门的一个物种，已知会引起毛霉病，但作为病毒宿主的了解甚少。在这里，我们使用琼脂糖凝胶电泳 (AGE) 和最近建立的碎片化和引物连接双链 RNA 测序 (FLDS) 方案，检查了 25 株临床 株的病毒感染情况。通过 AGE，检测到 5 株病毒感染株。然后，通过 FLDS 揭示了 12 种新型 RNA 病毒的全长基因组序列，这些病毒与家族 和单链 RNA (ssRNA) 病毒的未定义组的单链 RNA (ssRNA) 病毒有关，与已建立的家族 和拟议的家族“Ambiguiviridae”具有相似性。通过 FLDS 揭示了 12 种新型 RNA 病毒的全长基因组序列，除了一种具有负义 RNA 基因组的潜在 phasmavirid 外，所有这些特征病毒都具有正义 RNA 基因组。一种病毒属于家族内已建立的物种 ，而其他 11 种病毒则代表新的物种甚至新的属。这些结果表明，真菌病原体 携带多种 RNA 病毒，并扩展了我们对真菌门被囊动物、被囊动物门 Mucoromycota 中 RNA 病毒多样性的认识。从 的临床分离株中鉴定 RNA 病毒可能会在未来扩大毛霉病天然治疗剂的种类。
重要性：在被囊动物门真菌宿主中，真菌病毒的多样性被低估了，主要是在物种 中。此前，仅在该物种中发现了五个正义 RNA 基因组。由于目前的测序方法不能很好地完成基因组末端，我们使用碎片化和引物连接的双链 RNA 测序来获得全长基因组。在本研究中检测到 11 种新型真菌病毒，包括首次报道的负义 RNA 基因组 。我们的研究结果扩展了对 Mucoromycota 临床株病毒多样性的认识，可能为该真菌的发病机制和生态学提供了新的见解，并可能提供治疗选择。
