Key Laboratory of Microbial Signals and Disease Control, College of Plant Protection, South China Agricultural University, Guangzhou, Guangdong, China.
Front Cell Infect Microbiol. 2023 Aug 17;13:1229859. doi: 10.3389/fcimb.2023.1229859. eCollection 2023.
is a widespread edible ectomycorrhizal fungus that holds significant importance in both ecological and economic value. Mycoviruses are ubiquitous infectious agents hosted in different fungi, with some known to exert beneficial or detrimental effects on their hosts. However, mycoviruses hosted in ectomycorrhizal fungi remain poorly studied. To address this gap in knowledge, we employed next-generation sequencing (NGS) to investigate the virome of . Using BLASTp analysis and phylogenetic tree construction, we identified 33 mycovirus species, with over half of them belonging to the phylum , and 29 of these viruses were novel. These mycoviruses were further grouped into 11 lineages, with the discovery of a new negative-sense single-stranded RNA viral family in the order . In addition, our findings suggest the occurrence of cross-species transmission (CST) between the fungus and ticks, shedding light on potential evolutionary events that have shaped the viral community in different hosts. This study is not only the first study to characterize mycoviruses in but highlights the enormous diversity of mycoviruses and their implications for virus evolution.
是一种广泛分布的可食用外生菌根真菌，具有重要的生态和经济价值。真菌病毒是普遍存在于不同真菌中的传染性病原体，其中一些已知对其宿主有有益或有害的影响。然而，外生菌根真菌中的真菌病毒仍未得到充分研究。为了解决这一知识空白，我们采用下一代测序（NGS）技术来研究 的病毒组。通过 BLASTp 分析和系统发育树构建，我们鉴定出 33 种真菌病毒，其中超过一半属于 ，其中 29 种是新的。这些真菌病毒进一步分为 11 个谱系，在目 中发现了一种新的负义单链 RNA 病毒科。此外，我们的研究结果表明真菌和蜱之间存在跨物种传播（CST），这揭示了可能塑造不同宿主中病毒群落的潜在进化事件。本研究不仅首次对 中的真菌病毒进行了特征描述，还强调了真菌病毒的巨大多样性及其对病毒进化的影响。
