General Surgery Department, Beijing Friendship Hospital, Capital Medical University; National Clinical Research Center for Digestive Diseases; State Key Lab of Digestive Health, Beijing Friendship Hospital, Capital Medical University; Immunology Research Center for Oral and Systemic Health, Beijing Friendship Hospital, Capital Medical University; Beijing Laboratory of Oral Health, Capital Medical University School of Stomatology.
National Clinical Research Center for Digestive Diseases; State Key Lab of Digestive Health, Beijing Friendship Hospital, Capital Medical University; Immunology Research Center for Oral and Systemic Health, Beijing Friendship Hospital, Capital Medical University; Beijing Laboratory of Oral Health, Capital Medical University School of Stomatology; Beijing Clinical Research Institute.
J Vis Exp. 2024 Jul 12(209). doi: 10.3791/66465.
Most patients experience postoperative ileus (POI) after surgery, which is associated with increased morbidity, mortality, and hospitalization time. POI is a consequence of mechanical damage during surgery, resulting in disruption of motility in the gastrointestinal tract. The mechanisms of POI are related to aberrant neuronal sensitivity, impaired epithelial barrier function, and increased local inflammation. However, the details remain enigmatic. Therefore, experimental murine models are crucial for elucidating the pathophysiology and mechanism of POI injury and for the development of novel therapies. Here, we introduce a murine model of POI generated via intestinal manipulation (IM) that is similar to clinical surgery; this is achieved by mechanical damage to the small intestine by massaging the abdomen 1-3 times with a cotton swab. IM delayed gastrointestinal transit 24 h after surgery, as assessed by FITC-dextran gavage and fluorescence detection of the segmental digestive tract. Moreover, tissue swelling of the submucosa and immune cell infiltration were investigated by hematoxylin and eosin staining and flow cytometry. Proper pressure of the IM and a hyperemic effect on the intestine are critical for the procedure. This murine model of POI can be utilized to study the mechanisms of intestinal damage and recovery after abdominal surgery.
大多数患者在手术后都会经历术后肠梗阻（POI），这与发病率、死亡率和住院时间的增加有关。POI 是手术过程中机械损伤的结果，导致胃肠道蠕动中断。POI 的机制与异常神经元敏感性、上皮屏障功能受损和局部炎症增加有关。然而，其细节仍然难以捉摸。因此，实验性小鼠模型对于阐明 POI 损伤的病理生理学和机制以及开发新的治疗方法至关重要。在这里，我们介绍了一种通过肠道操作（IM）产生的类似于临床手术的 POI 小鼠模型；这是通过用棉签对腹部进行 1-3 次按摩来对小肠造成机械损伤来实现的。通过 FITC-葡聚糖灌胃和荧光检测节段性消化道，术后 24 小时评估 IM 延迟了胃肠道转运。此外，通过苏木精和曙红染色和流式细胞术研究了黏膜下层组织肿胀和免疫细胞浸润。适当的 IM 压力和肠道的充血效应对该过程至关重要。这种 POI 小鼠模型可用于研究腹部手术后肠道损伤和恢复的机制。
