Benramdane Hanane, Benariba Nabila, Silva Carlos F M, Catarino Marcelo D, Bartolomeu Maria, Fekhikher Zohra, Pinto Diana C G A
LAPSAB, Department of Biology, University of Abou Bekr Belkaid, Tlemcen, 13000, Algeria.
LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, Aveiro, 3810-193, Portugal.
Chem Biodivers. 2024 Dec;21(12):e202401308. doi: 10.1002/cbdv.202401308. Epub 2024 Sep 14.
Peganum harmala seeds crude hydro-methanolic extract and their fractions (obtained with ethyl acetate and butan-1-ol) were analyzed and compared for their chemical profiles of alkaloids and polyphenols content. Moreover, their antioxidant, α-glucosidase, acetylcholinesterase, and butyrylcholinesterase inhibitory activities were evaluated. The butan-1-ol fraction revealed the highest total phenolic content and exhibited the highest antioxidant capacity. From the inhibitory enzyme evaluations, it should be highlighted the butan-1-ol fraction inhibitory potential of ɑ-glucosidase (the IC=141.18±4 μg/mL), which was better than the acarbose inhibitory effect (IC=203.41±1.07 μg/mL). The extracts' chemical profile analysis revealed several compounds, in which quercetin dimethyl ether, harmine and harmaline emerged as the major compounds. The different solvents used impacted Peganum harmala seed contents and biological responses. Statistical analysis showed a significant correlation between bioactive compounds and biological activities. Thus, Peganum harmala seeds could be a promising natural source of bioactive compounds at the crossroads of many human diseases, and its cultivation may be encouraged.
对骆驼蓬种子的粗水甲醇提取物及其馏分（用乙酸乙酯和丁醇-1获得）进行了分析，并比较了它们生物碱和多酚含量的化学特征。此外，还评估了它们的抗氧化、α-葡萄糖苷酶、乙酰胆碱酯酶和丁酰胆碱酯酶抑制活性。丁醇-1馏分显示出最高的总酚含量，并表现出最高的抗氧化能力。从抑制酶的评估中可以看出，丁醇-1馏分对α-葡萄糖苷酶的抑制潜力（IC=141.18±4μg/mL）优于阿卡波糖的抑制效果（IC=203.41±1.07μg/mL）。提取物的化学特征分析揭示了几种化合物，其中槲皮素二甲醚、骆驼蓬碱和去氢骆驼蓬碱是主要化合物。使用的不同溶剂影响了骆驼蓬种子的成分和生物学反应。统计分析表明生物活性化合物与生物活性之间存在显著相关性。因此，骆驼蓬种子可能是许多人类疾病交叉点上有前景的生物活性化合物天然来源，可能会鼓励其种植。
