Laboratory of Physiology, Faculty of Medicineof Tunis, University of Tunis El Manar, Tunis, La Rabta, 1007, Tunisia.
Laboratory of Physiopathology, Food and Biomolecules (LR-17-ES-03), Technology Center of Sidi Thabet, University of Manouba, Tunis, Tunisa.
Chem Biodivers. 2024 Nov;21(11):e202401030. doi: 10.1002/cbdv.202401030. Epub 2024 Sep 13.
Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive interstitial disease leading to pulmonary damage and respiratory failure. We aimed to investigate the effect of prickly pear molasses (PPM) on an experimental model of pulmonary fibrosis induced by bleomycin (BLM) in Wistar rat. Animals were divided into 5 groups: the control group (G1), the BLM group (G2) and three groups (G3, G4, G5) receiving a single intra-tracheal injection of BLM (4 mg/kg) and PPM (at 2, 4.5 and10 %) that was introduced into the diet one week before BLM injection and continued for 3 weeks. Our phytochemical results revealed significant polyphenol and flavonoid content. LCMS analysis revealed the presence of Sinapinic acid, t-ferulic acid, t-cinnamic acid, Caffeic acid, gallic acid and vallinic acid among others. Our histological study revealed significant decrease in collagen deposition in the groups of rats treated with 4.5 % and 10 % molasses compared to BLM group. Oxidative stress in pulmonary tissues was investigated using catalase (CAT), superoxide dismutase (SOD), and malondialdehyde (MDA) assays. Treatment with PPM normalized the disturbance in the level of these oxidative markers in G3,G4, G5 compared to G2. In conclusion, PPM exhibit antifibrotic and antioxidant activities in BLM model of lung fibrosis.
特发性肺纤维化（IPF）是一种慢性进行性间质性疾病，导致肺损伤和呼吸衰竭。我们旨在研究刺梨蜜（PPM）对博莱霉素（BLM）诱导的 Wistar 大鼠肺纤维化实验模型的影响。动物分为 5 组：对照组（G1）、BLM 组（G2）和三组（G3、G4、G5），每组大鼠接受单次气管内注射 BLM（4mg/kg）和 PPM（2%、4.5%和 10%），PPM 于 BLM 注射前一周引入饮食中，并持续 3 周。我们的植物化学结果显示出显著的多酚和类黄酮含量。LCMS 分析显示存在芥子酸、反式-阿魏酸、反式-肉桂酸、咖啡酸、没食子酸和缬草酸等。我们的组织学研究表明，与 BLM 组相比，用 4.5%和 10%蜜糖水治疗的大鼠的胶原蛋白沉积显著减少。使用 CAT、SOD 和 MDA 测定法研究肺组织中的氧化应激。与 G2 相比，PPM 治疗使 G3、G4 和 G5 中这些氧化标记物水平的紊乱正常化。总之，PPM 在 BLM 诱导的肺纤维化模型中表现出抗纤维化和抗氧化活性。
