Department of Biochemistry, Cell & Molecular Biology/West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana.
Department of Medical Laboratory Sciences, University of Ghana, Accra, Ghana.
Kaohsiung J Med Sci. 2024 Sep;40(9):830-836. doi: 10.1002/kjm2.12880. Epub 2024 Jul 29.
Human papillomavirus (HPV)-related cervical and nasopharyngeal cancers differ in molecular mechanisms underlying the oncogenic processes. The disparity may be attributed to differential expression of oncoproteins. The current study investigated the host oncogenes expression pattern in HPV-associated cervical and nasopharyngeal cancer. Formalin-fixed paraffin-embedded tissues originating from the nasopharyngeal and cervical regions were screened using Hematoxylin and Eosin staining. Genomic DNA and total RNA were extracted from confirmed cancer biopsies and non-cancer tissues (NC). HPV was detected by PCR using MY09/GP5+/6+ primers. Protein expression levels of AKT, IQGAP1, and MMP16 in HPV-infected cancers and controls were determined by immunohistochemistry. RT-qPCR was used to profile mRNAs of the oncogenes. AKT and IQGAP1 proteins were highly expressed in the epithelial cancers compared with the non-cancer tissues (p < 0.05). IQGAP1 and MMP16 mRNAs level was significantly higher in the cancers than in the NC (p < 0.05), but not AKT mRNA levels. MMP16 protein was ubiquitously expressed in all tissues. AKT mRNA level was significantly elevated in CC compared with NPC (p < 0.001). However, the difference in AKT, IQGAP1 and MMP16 proteins level between CC and NPC was not significant (p > 0.05). The oncoproteins expression level between the HPV-positive and HPV-negative cancer biopsies showed no significant difference (p < 0.05). Current study reports AKT but not IQGAP1 and MMP16 mRNAs differentially expression in cervical and nasopharyngeal cancers, independent of HPV infection status.
人乳头瘤病毒（HPV）相关的宫颈和鼻咽癌症在致癌过程中的分子机制上有所不同。这种差异可能归因于癌蛋白的差异表达。本研究调查了 HPV 相关宫颈和鼻咽癌症中宿主癌基因的表达模式。使用苏木精和伊红染色对来自鼻咽和宫颈区域的福尔马林固定石蜡包埋组织进行筛选。从确诊的癌症活检和非癌症组织（NC）中提取基因组 DNA 和总 RNA。使用 MY09/GP5+/6+引物通过 PCR 检测 HPV。通过免疫组织化学法测定 HPV 感染癌症和对照中 AKT、IQGAP1 和 MMP16 的蛋白表达水平。使用 RT-qPCR 分析癌基因的 mRNA 谱。与非癌症组织相比，上皮性癌症中 AKT 和 IQGAP1 蛋白表达水平更高（p<0.05）。与 NC 相比，癌症中的 IQGAP1 和 MMP16 mRNA 水平显著更高（p<0.05），但 AKT mRNA 水平没有差异。MMP16 蛋白在所有组织中均广泛表达。与 NPC 相比，CC 中的 AKT mRNA 水平显著升高（p<0.001）。然而，CC 和 NPC 之间 AKT、IQGAP1 和 MMP16 蛋白水平的差异无统计学意义（p>0.05）。HPV 阳性和 HPV 阴性癌症活检中癌蛋白的表达水平无显著差异（p<0.05）。本研究报告了 AKT 而不是 IQGAP1 和 MMP16 mRNA 在宫颈和鼻咽癌症中的差异表达，与 HPV 感染状态无关。
