Knecht E A, Moorman W J, Clark J C, Lynch D W, Lewis T R
Am Rev Respir Dis. 1985 Dec;132(6):1181-5. doi: 10.1164/arrd.1985.132.6.1181.
An experimental study was conducted to investigate the hypothesis that changes in pulmonary function induced by vanadium pentoxide (V2O5) inhalation would be accompanied by evidence of pulmonary inflammation. Sixteen adult, male cynomolgus monkeys were acutely exposed by whole-body inhalation of V2O5 dust at aerosol concentrations of 0.5 mg V2O5/m3 and 5.0 mg V2O5/m3, conducted at a 1-wk interval. Comprehensive pulmonary function tests were performed 1 day after each inhalation exposure to detect functional changes in the airways and pulmonary parenchyma. Pulmonary inflammation was assessed by cytologic analysis of respiratory cells recovered from the lower respiratory tract by bronchoalveolar lavage (BAL). Postexposure values for pulmonary function and BAL were compared with the baseline values determined for each monkey prior to V2O5 exposure. Acute V2O5 dust inhalation produced significant air-flow limitation in both central and peripheral airways without producing any detectable changes in parenchymal function. These functional changes were accompanied by a significant increase in the total cell counts recovered from the lungs by BAL. The increase in total cell count occurred through a dramatic increase in absolute number and relative percentage of polymorphonuclear leukocytes (PMN). These findings suggest that pulmonary inflammatory changes involving PMN may play an important role in the occurrence of air-flow limitation after acute inhalation of V2O5 dust.
进行了一项实验研究，以调查以下假设：吸入五氧化二钒（V2O5）引起的肺功能变化会伴有肺部炎症迹象。16只成年雄性食蟹猴以0.5毫克V2O5/立方米和5.0毫克V2O5/立方米的气溶胶浓度，通过全身吸入V2O5粉尘进行急性暴露，暴露间隔为1周。在每次吸入暴露后1天进行全面的肺功能测试，以检测气道和肺实质的功能变化。通过对支气管肺泡灌洗（BAL）从下呼吸道回收的呼吸细胞进行细胞学分析来评估肺部炎症。将暴露后的肺功能和BAL值与每只猴子在V2O5暴露前测定的基线值进行比较。急性吸入V2O5粉尘在中央和外周气道均产生了显著的气流受限，而肺实质功能未出现任何可检测到的变化。这些功能变化伴随着通过BAL从肺部回收的总细胞数显著增加。总细胞数的增加是通过多形核白细胞（PMN）的绝对数量和相对百分比急剧增加而发生的。这些发现表明，涉及PMN的肺部炎症变化可能在急性吸入V2O5粉尘后气流受限的发生中起重要作用。
