Al-Saadi Hayder, Potapova Inga, Rochford Edward Tj, Moriarty Thomas F, Messmer Peter
AO Research Institute Davos, AO Foundation, Davos Platz, Switzerland.
Traumatologie Klinik St. Anna, Luzern, Switzerland.
Int Wound J. 2016 Oct;13(5):936-42. doi: 10.1111/iwj.12412. Epub 2015 Jan 14.
Infections associated with deep wounds require extensive surgical and medical care. New adjunctive treatments are required to aid in the eradication of the bacterial biofilms found on infected wounds and, in particular, any underlying hardware. Ozone has been used as a safe and efficient disinfectant in water treatment plants for many years. The purpose of this study is to investigate the anti-biofilm potential of ozonated saline against biofilms of Staphylococcus aureus, a microorganism commonly implicated in wound infections. A custom-made bacterial biofilm bioreactor was used to grow S. aureus biofilms on discs of medical grade titanium alloy. An ozone generator was connected in-line and biofilms and planktonic bacteria were exposed to ozone in saline. Cytotoxicity was assessed against primary ovine osteoblasts in the same system. In tests against planktonic S. aureus, a 99% reduction in bacterial numbers was detected within 15 minutes of exposure. S. aureus biofilms were significantly more resistant to ozone, although complete eradication of the biofilm was eventually achieved within 5 hours. Ozonated saline was not found to be cytotoxic to primary ovine osteoblasts. Ozonated saline may be suitable as an adjuvant therapy to treat patients as an instillation fluid for wound irrigation and sterilisation.
与深部伤口相关的感染需要广泛的外科和医疗护理。需要新的辅助治疗方法来帮助根除感染伤口上发现的细菌生物膜，尤其是任何潜在的植入物上的生物膜。臭氧多年来一直被用作水处理厂的安全高效消毒剂。本研究的目的是调查臭氧盐水对金黄色葡萄球菌生物膜的抗生物膜潜力，金黄色葡萄球菌是一种常见的与伤口感染有关的微生物。使用定制的细菌生物膜生物反应器在医用级钛合金圆盘上培养金黄色葡萄球菌生物膜。在线连接臭氧发生器，使生物膜和浮游细菌暴露于盐水中的臭氧。在同一系统中评估对原代绵羊成骨细胞的细胞毒性。在针对浮游金黄色葡萄球菌的测试中，暴露15分钟内细菌数量减少了99%。金黄色葡萄球菌生物膜对臭氧的抵抗力明显更强，尽管最终在5小时内实现了生物膜的完全根除。未发现臭氧盐水对原代绵羊成骨细胞具有细胞毒性。臭氧盐水可能适合作为辅助治疗方法，作为伤口冲洗和消毒的滴注液来治疗患者。
