Department of Ophthalmology, Hebei Medical University, Shijiazhuang, 050017, Hebei, China; Hebei Eye Hospital, Number 399 Quanbei East Street, Xingtai, 054001, Hebei, China.
Department of Ophthalmology, Hebei Medical University, Shijiazhuang, 050017, Hebei, China; Hebei Eye Hospital, Number 399 Quanbei East Street, Xingtai, 054001, Hebei, China; Hebei Provincial Key Laboratory of Ophthalmology, Xingtai, 054001, Hebei, China; Hebei Provincial Clinical Research Center for Eye Diseases, Xingtai, 054001, Hebei, China.
Exp Eye Res. 2024 Sep;246:110014. doi: 10.1016/j.exer.2024.110014. Epub 2024 Jul 27.
In this study, we investigated the effects of flickering light on refractive development of mice and the changes of fundus structure and function during this process. C57BL/6 mice were randomly divided into control group and flickering light-induced myopia (FLM) group. Mice in the control group were fed under normal lighting. FLM group mice were fed under lighting with a duty cycle of 50% and flash frequency of 2 Hz. Refractive status, axial length (AL), corneal radius of curvature (CRC), and electroretinogram signals were measured in all animals before treatment and at 2 and 4 weeks after treatment. Retinal thickness (RT), choroidal thickness (ChT) and choroidal blood perfusion (ChBP) were measured by optical coherence tomography (OCT) and optical coherence tomography angiography (OCTA). After 4 weeks of flickering light stimulation, the mice became myopia, the AL increased, but the CRC remained constant. The induction of myopia reduced the implicit time and amplitude of a-wave and b-wave in electroretinogram, which affects the function of retina. Full-layer retinal thickness, ChT and ChBP decreased at both 2 and 4 weeks after flickering light induction. The superficial and middle layers of the retina were significantly thinner, while the deep layer was only slightly thinner without statistical significance. Calculated by the concentric circle algorithm, the decrease of choroidal blood perfusion in FLM was mainly concentrated in the concentric circle area with the optic disc as the center radius of 150-450 μm. In conclusion, the present study shows that flickering light can successfully induce myopia in C57BL/6 mice, affect the electrophysiological activity of retina, and cause changes in fundus tissue structure and blood flow.
在这项研究中，我们研究了闪烁光对小鼠屈光发育的影响，以及在此过程中眼底结构和功能的变化。C57BL/6 小鼠被随机分为对照组和闪烁光诱导近视（FLM）组。对照组小鼠在正常光照下饲养。FLM 组小鼠在光照下饲养，光照周期为 50%，闪烁频率为 2 Hz。所有动物在治疗前和治疗后 2 周和 4 周时测量屈光状态、眼轴（AL）、角膜曲率半径（CRC）和视网膜电图信号。采用光学相干断层扫描（OCT）和光学相干断层扫描血管造影（OCTA）测量视网膜厚度（RT）、脉络膜厚度（ChT）和脉络膜血流灌注（ChBP）。经过 4 周的闪烁光刺激后，小鼠出现近视，AL 增加，但 CRC 保持不变。近视的诱导降低了视网膜电图中 a 波和 b 波的潜伏期和振幅，从而影响了视网膜的功能。全层视网膜厚度、ChT 和 ChBP 在闪烁光诱导后 2 周和 4 周均下降。视网膜浅层和中层变薄更明显，而深层只有轻微变薄，无统计学意义。用同心圆算法计算，FLM 脉络膜血流灌注的减少主要集中在以视盘为中心、半径为 150-450 μm 的同心圆区域。总之，本研究表明，闪烁光可以成功诱导 C57BL/6 小鼠发生近视，影响视网膜的电生理活动，并导致眼底组织结构和血流发生变化。
