Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China; College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
Key Laboratory of Environment Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China; College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China; Key Laboratory of Water Pollution Control and Environmental Safety of Zhejiang Province, Hangzhou, 310058, China.
Environ Pollut. 2024 Nov 1;360:124628. doi: 10.1016/j.envpol.2024.124628. Epub 2024 Jul 27.
Arsenic (As) and polycyclic aromatic hydrocarbons (PAHs) are highly toxic, carcinogenic and teratogenic, and are commonly found in soils of industrial sites such as coking plants. They exert environmental stresses on soil microorganisms, but their compounding effects have not been systematically studied. Exploring the effects of compound contamination on microbial communities, species and genes is important for revealing the ecological damage caused by compound contamination and offering scientific insights into soil remediation strategies. In this study, we selected soil samples from 0 to 100 cm depth of a coking site with As, PAHs and compound contamination. We investigated the compound effects of As and PAHs on microbial communities by combining high-throughput sequencing, metagenomic sequencing and genome assembly. Compared with single contamination, compound contamination reduced the microbial community diversity by 10.68%-12.07% and reduced the community richness by 8.39%-18.61%. The compound contamination decreased 32.41%-46.02% of microbial PAHs metabolic gene abundance, 11.36%-19.25% of cell membrane transport gene abundance and 12.62%-57.77% of cell motility gene abundance. Xanthobacteraceae, the biomarker for compound contaminated soils, harbors arsenic reduction genes and PAHs degradation pathways of naphthalene, benzo [a]pyrene, fluorene, anthracene, and phenanthrene. Its broad metabolic capabilities, encompassing sulfur metabolism and quorum sensing, facilitate the acquisition of energy and nutrients, thereby conferring ecological niche advantages in compound contaminated environments. This study underscores the significant impacts of As and PAHs on the composition and function of microbial communities, thereby enriching our understanding of their combined effects and providing insights for the remediation of compound contaminated sites.
砷（As）和多环芳烃（PAHs）是高毒性、致癌和致畸的物质，通常存在于焦化厂等工业场地的土壤中。它们对土壤微生物施加环境压力，但它们的复合效应尚未得到系统研究。探索复合污染对微生物群落、物种和基因的影响，对于揭示复合污染造成的生态破坏，为土壤修复策略提供科学见解具有重要意义。在这项研究中，我们选择了焦化场地 0-100cm 深度的土壤样本，这些土壤样本受到 As、PAHs 和复合污染的影响。我们通过结合高通量测序、宏基因组测序和基因组组装，研究了 As 和 PAHs 对微生物群落的复合效应。与单一污染相比，复合污染使微生物群落多样性降低了 10.68%-12.07%，丰富度降低了 8.39%-18.61%。复合污染使微生物 PAHs 代谢基因丰度降低了 32.41%-46.02%，细胞膜转运基因丰度降低了 11.36%-19.25%，细胞运动基因丰度降低了 12.62%-57.77%。黄杆菌科是复合污染土壤的生物标志物，它含有砷还原基因和萘、苯并[a]芘、芴、蒽和菲的 PAHs 降解途径。它广泛的代谢能力，包括硫代谢和群体感应，使其能够获得能量和营养物质，从而在复合污染环境中具有生态位优势。这项研究强调了 As 和 PAHs 对微生物群落组成和功能的重大影响，丰富了我们对它们复合效应的理解，并为复合污染场地的修复提供了见解。
