Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong 266237, China; Suzhou Research Institute, Shandong University, Suzhou, Jiangsu 215123, China.
Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong 266237, China.
Sci Total Environ. 2021 Sep 10;786:147338. doi: 10.1016/j.scitotenv.2021.147338. Epub 2021 Apr 26.
Both potentially toxic elements (PTEs) and polycyclic aromatic hydrocarbons (PAHs) are widely present in soil contaminated by steel industries. This study assessed the vertical variation (at 20 cm, 40 cm, 60 cm, 80 cm, 120 cm, and 150 cm depth) of bacterial abundance, community structure, functional genes related to PAHs degradation, and community co-occurrence patterns in an old steel plant soils which contaminated by PTEs and PAHs for 60 years. The excessive PAHs and PTEs in steel plant soils were benzo (a) pyrene, benzo (b) fluoranthene, dibenzo (a, h) anthracene, indeno (1,2,3-c, d) pyrene, and lead (Pb). The abundance and composition of bacterial community considerably changed with soil depth in two study areas with different pollution degrees. The results of co-occurrence network analysis indicated that the top genera in blast furnace zone identified as the potential keystone taxa were Haliangium, Blastococcus, Nitrospira, and Sulfurifustis. And in coking zone, the top genera were Gaiella. The predictions of bacterial metabolism function using PICRUSt showed that the PAHs-PTEs contaminated soil still had the potential for PAHs degradation, but most PTEs negatively correlated with PAHs degradation genes. The total sulfur (TS), acenaphthene (ANA), and Zinc (Zn) were the key factors to drive development of bacterial communities in the steel plant soils. As far as we know, this is the first investigation of vertical distribution and interaction of the bacterial microbiota in the aging soils of steel plant contaminated with PTEs and PAHs.
潜在有毒元素 (PTEs) 和多环芳烃 (PAHs) 广泛存在于受钢铁工业污染的土壤中。本研究评估了受 PTEs 和 PAHs 污染 60 年的老钢铁厂土壤中细菌丰度、群落结构、与 PAHs 降解相关的功能基因以及群落共生模式的垂直变化（在 20 cm、40 cm、60 cm、80 cm、120 cm 和 150 cm 深度）。钢铁厂土壤中过量的 PAHs 和 PTEs 为苯并（a）芘、苯并（b）荧蒽、二苯并（a, h）蒽、茚并（1,2,3-c, d）芘和铅（Pb）。两个污染程度不同的研究区域中，土壤深度对细菌群落的丰度和组成有很大影响。共生网络分析结果表明，高炉区的优势属鉴定为潜在的关键类群，包括 Haliangium、Blastococcus、Nitrospira 和 Sulfurifustis。在炼焦区，优势属为 Gaiella。使用 PICRUSt 预测细菌代谢功能表明，PAHs-PTEs 污染土壤仍具有 PAHs 降解潜力，但大多数 PTEs 与 PAHs 降解基因呈负相关。总硫 (TS)、苊 (ANA) 和锌 (Zn) 是驱动钢铁厂土壤中细菌群落发展的关键因素。据我们所知，这是首次对受 PTEs 和 PAHs 污染的老化钢铁厂土壤中细菌微生物群落的垂直分布和相互作用进行调查。
