Graduate School of Science and Engineering, Ibaraki University, Hitachi, Ibaraki 316-8511, Japan; College of Vocational Studies, IPB University, Cilibende, Bogor, West Java 16128, Indonesia.
Graduate School of Science and Engineering, Ibaraki University, Hitachi, Ibaraki 316-8511, Japan.
Sci Total Environ. 2024 Nov 1;949:175089. doi: 10.1016/j.scitotenv.2024.175089. Epub 2024 Jul 27.
Using structural equation modeling (SEM), we investigated multiple biomarker mechanisms in terms of biochemical and individual marker responses in the brackish water clam Corbicula japonica following acute exposure to polystyrene microplastic (PS-MP). This study is the first to comprehensively explore multiple biomarker responses in bivalves using SEM. The model revealed that PS-MP accumulation was an independent biomarker, exhibiting significant direct effects on superoxide dismutase (SOD) and catalase (CAT) among the biochemical markers. Although CAT generally interacts closely with SOD, no significant relationship was identified between them, indicating that CAT may have independently responded to PS-MP stress. Among individual markers, significant indirect effects were observed on clearance rate (CR), reflecting feeding activity and valve open rate, indicating excretion activity via SOD and CAT. Finally, the carbon-based scope for growth was significantly influenced by CR. SEM is efficient and useful for identifying significant direct and indirect pathway relationships and for uncovering uncommon relationships in unified multiple biomarker mechanisms in aquatic studies.
采用结构方程模型（SEM），我们研究了在咸水蛤日本鲍急性暴露于聚苯乙烯微塑料（PS-MP）后，生物化学和个体标志物反应方面的多种生物标志物机制。本研究首次使用 SEM 全面探讨了双壳类动物的多种生物标志物反应。该模型表明，PS-MP 积累是一个独立的生物标志物，对生物化学标志物中的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）表现出显著的直接影响。尽管 CAT 通常与 SOD 密切相关，但它们之间没有发现显著关系，这表明 CAT 可能已经独立地对 PS-MP 应激做出了反应。在个体标志物中，清除率（CR）表现出显著的间接影响，反映了摄食活性和瓣开合率，表明通过 SOD 和 CAT 进行排泄活动。最后，碳基生长范围受到 CR 的显著影响。SEM 可有效用于识别重要的直接和间接途径关系，并揭示水生研究中统一的多种生物标志物机制中的不常见关系。
