Unit of Microbiology, Department of Life and Environmental Sciences, Polytechnic University of Marche, 60131 Ancona, Italy.
Unit of Microbiology, Department of Biomedical Sciences and Public Health, Polytechnic University of Marche Medical School, Via Tronto 10/A, 60126 Ancona, Italy.
J Appl Microbiol. 2024 Aug 5;135(8). doi: 10.1093/jambio/lxae193.
To investigate enterococci carrying linezolid and vancomycin resistance genes from fecal samples recovered from wild boars.
Florfenicol- and vancomycin-resistant enterococci, isolated on selective agar plates, were screened by PCR for the presence of linezolid and vancomycin resistance genes. Five isolates carried optrA or poxtA linezolid resistance genes; one strain was resistant to vancomycin for the presence of vanA gene. All isolates were tested for their antibiotic susceptibility and subjected to Whole Genome Sequencing (WGS) analysis. In Enterococcus faecalis (E. faecalis) V1344 and V1676, the optrA was located on the new pV1344-optrA and pV1676-optrA plasmids, respectively, whereas in Enterococcus faecium (E. faecium) V1339 this gene was on a 22 354-bp chromosomal genetic context identical to the one detected in a human E. faecium isolate. In both E. faecium V1682 and E. durans V1343, poxtA was on the p1818-c plasmid previously found in a human E. faecium isolate. In E. faecium V1328, the vanA gene was on the Tn1546 transposon in turn located on a new pV1328-vanA plasmid. Only E. faecium V1682 successfully transferred the poxtA gene to an enterococcal recipient in filter mating assays.
The occurrence of genetic elements carrying linezolid and vancomycin resistance genes in enterococci from wild boars is a matter of concern, moreover, the sharing of plasmids and transposons between isolates from wild animals, human, and environment indicates an exchange of genetic material between these settings.
从野猪粪便样本中调查携带利奈唑胺和万古霉素耐药基因的肠球菌。
在选择性琼脂平板上分离出的氟苯尼考和万古霉素耐药肠球菌，通过 PCR 筛选携带利奈唑胺和万古霉素耐药基因。有 5 株分离株携带 optrA 或 poxtA 利奈唑胺耐药基因；1 株对万古霉素耐药，其携带 vanA 基因。所有分离株均进行了抗生素敏感性测试，并进行了全基因组测序（WGS）分析。在屎肠球菌（E. faecalis）V1344 和 V1676 中，optrA 分别位于新的 pV1344-optrA 和 pV1676-optrA 质粒上，而在屎肠球菌（E. faecium）V1339 中，该基因位于与从人类屎肠球菌分离株中检测到的相同的 22354bp 染色体遗传背景上。在屎肠球菌 V1682 和缓症肠球菌（E. durans）V1343 中，poxtA 位于之前在人类屎肠球菌分离株中发现的 p1818-c 质粒上。在屎肠球菌 V1328 中，vanA 基因位于 Tn1546 转座子上，该转座子又位于新的 pV1328-vanA 质粒上。只有屎肠球菌 V1682 在滤膜交配试验中成功地将 poxtA 基因转移到肠球菌受体中。
从野猪肠球菌中发现携带利奈唑胺和万古霉素耐药基因的遗传元件令人担忧，此外，从野生动物、人类和环境中分离出的菌株之间共享质粒和转座子表明这些环境之间存在遗传物质的交换。
