电荷复合动力学作为光合反应中心初级受体质子化的一种探测手段。
Charge recombination kinetics as a probe of protonation of the primary acceptor in photosynthetic reaction centers.
作者信息
Kleinfeld D, Okamura M Y, Feher G
出版信息
Biophys J. 1985 Nov;48(5):849-52. doi: 10.1016/S0006-3495(85)83844-3.
Abstract
The kinetics of the charge recombination D+QA-----DQA was used to probe the protonation of the primary acceptor in reaction centers from Rhodopseudomonas sphaeroides, in which the native ubiquinone was replaced by anthraquinone. We found that QA- is stabilized by the rapid (t less than 10(-2) s) binding of a proton, with a pK of 9.8. The distance between QA- and the proton binding site was estimated to be larger than approximately 5 A.
摘要
利用电荷复合D+QA-----DQA的动力学来探测球形红假单胞菌反应中心中初级受体的质子化情况，其中天然泛醌被蒽醌取代。我们发现QA-通过质子的快速（t小于10^(-2)秒）结合而稳定，其pK为9.8。估计QA-与质子结合位点之间的距离大于约5埃。
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本文引用的文献
1
Charged amino acids as spectroscopic determinants for chlorophyll in vivo.
Proc Natl Acad Sci U S A. 1983 Aug;80(16):4959-62. doi: 10.1073/pnas.80.16.4959.
2
Stability of "salt bridges" in membrane proteins.
Proc Natl Acad Sci U S A. 1984 Sep;81(17):5412-6. doi: 10.1073/pnas.81.17.5412.
3
Macroscopic models for studies of electrostatic interactions in proteins: limitations and applicability.
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4785-9. doi: 10.1073/pnas.81.15.4785.
4
Electron transfer in reaction centers of Rhodopseudomonas sphaeroides. I. Determination of the charge recombination pathway of D+QAQ(-)B and free energy and kinetic relations between Q(-)AQB and QAQ(-)B.
Biochim Biophys Acta. 1984 Jul 27;766(1):126-40. doi: 10.1016/0005-2728(84)90224-x.
5
Direct measurement of the redox potential of the primary and secondary quinone electron acceptors in Rhodopseudomonas sphaeroides (wild-type) by EPR spectrometry.
FEBS Lett. 1980 Feb 11;110(2):257-61. doi: 10.1016/0014-5793(80)80086-x.
6
Calculations of electrostatic interactions in biological systems and in solutions.
Q Rev Biophys. 1984 Aug;17(3):283-422. doi: 10.1017/s0033583500005333.
7
Optical and kinetic properties of semireduced plastoquinone and ubiquinone: electron acceptors in photosynthesis.
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8
Electron acceptors in reaction center preparations from photosynthetic bacteria.
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9
Photochemical electron transport in photosynthetic reaction centers. IV. Observations related to the reduced photoproducts.
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Light induced H+ uptake catalysed by photochemical reaction centres from Rhodopseudomonas spheroides R26.
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