电荷复合动力学作为光合反应中心初级受体质子化的一种探测手段。
Charge recombination kinetics as a probe of protonation of the primary acceptor in photosynthetic reaction centers.
作者信息
Kleinfeld D, Okamura M Y, Feher G
出版信息
Biophys J. 1985 Nov;48(5):849-52. doi: 10.1016/S0006-3495(85)83844-3.
Abstract
The kinetics of the charge recombination D+QA-----DQA was used to probe the protonation of the primary acceptor in reaction centers from Rhodopseudomonas sphaeroides, in which the native ubiquinone was replaced by anthraquinone. We found that QA- is stabilized by the rapid (t less than 10(-2) s) binding of a proton, with a pK of 9.8. The distance between QA- and the proton binding site was estimated to be larger than approximately 5 A.
摘要
利用电荷复合D+QA-----DQA的动力学来探测球形红假单胞菌反应中心中初级受体的质子化情况，其中天然泛醌被蒽醌取代。我们发现QA-通过质子的快速（t小于10^(-2)秒）结合而稳定，其pK为9.8。估计QA-与质子结合位点之间的距离大于约5埃。
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