Samioti Venetia, Kriti Evangelia, Spanou Aikaterini, Tsironi Theofania, Panagou Efstathios Z
Laboratory of Microbiology and Biotechnology of Foods Department of Food Science and Human Nutrition School of Food and Nutritional Sciences Agricultural University of Athens, Iera Odos 75, Athens GR-11855, Greece.
Laboratory of Food Process Engineering Department of Food Science and Human Nutrition School of Food and Nutritional Sciences Agricultural University of Athens, Iera Odos 75, Athens GR-11855, Greece.
Int J Food Sci. 2024 Jul 22;2024:7464133. doi: 10.1155/2024/7464133. eCollection 2024.
In the present study, selected pathogenic ( Typhimurium, , , , , and ) and nonpathogenic (, , , , , and ) bacteria were subjected in vitro in cold atmospheric pressure plasma (CAPP) treatment for up to 15 min and the changes in the surviving microbial population were determined. Plasma treatments were carried out by a plasma jet device, operating with argon (Ar) as carrier gas under constant flow (4.0 L/min) at a frequency of 1 MHz and an electrical voltage of 2-6 kV. Microbial inactivation data were modelled using linear and nonlinear (Geeraerd, Weibull) models, through which the corresponding kinetic parameters were calculated. After 15 min of exposure to plasma radiation, the total reduction in the bacterial populations was 2.12 log CFU mL for , 1.77 log CFU mL for , 2.30 log CFU mL for , 1.58 log CFU mL for , 1.31 log CFU mL for , 3.80 log CFU mL for (highest reduction observed), 1.12 log CFU mL for . Typhimurium, 1.18 log CFU mL for , 1.43 log CFU mL for , 1.32 log CFU mL for , 0.88 log CFU mL for , and 0.73 log CFU mL for . The results showed a higher reduction in the population of nonpathogenic microorganisms compared to pathogens. The relatively small decrease in the inactivation of bacteria indicates that parameter optimization is necessary to be considered to improve the efficacy of the treatment.
在本研究中，选取了致病性细菌（鼠伤寒沙门氏菌等）和非致病性细菌（等），使其在体外接受冷大气压等离子体（CAPP）处理长达15分钟，并测定存活微生物数量的变化。等离子体处理通过等离子体射流装置进行，以氩气（Ar）作为载气，在恒定流量（4.0升/分钟）下，频率为1兆赫兹，电压为2 - 6千伏。微生物失活数据使用线性和非线性（吉拉德、韦布尔）模型进行建模，通过这些模型计算相应的动力学参数。暴露于等离子体辐射15分钟后，细菌数量的总减少量分别为：[细菌名称1]为2.12 log CFU/mL，[细菌名称2]为1.77 log CFU/mL，[细菌名称3]为2.30 log CFU/mL，[细菌名称4]为1.58 log CFU/mL，[细菌名称5]为1.31 log CFU/mL，[细菌名称6]为3.80 log CFU/mL（观察到的最大减少量），[细菌名称7]为1.12 log CFU/mL。鼠伤寒沙门氏菌为1.18 log CFU/mL，[细菌名称8]为1.43 log CFU/mL，[细菌名称9]为1.32 log CFU/mL，[细菌名称10]为0.88 log CFU/mL，[细菌名称11]为0.73 log CFU/mL。结果表明，与致病性微生物相比，非致病性微生物数量的减少幅度更大。细菌失活的相对较小降幅表明，需要考虑参数优化以提高处理效果。
