Kilonzo-Nthenge Agnes, Liu Siqin, Yannam Sudheer, Patras Ankit
Department of Human Sciences, Tennessee State University, Nashville, TN, United States.
Department of Agricultural and Environmental Sciences, College of Agriculture, Tennessee State University, Nashville, TN, United States.
Front Nutr. 2018 Dec 11;5:120. doi: 10.3389/fnut.2018.00120. eCollection 2018.
The contamination of fruits with human pathogens is a reoccurring concern in the fresh produce industry. Atmospheric cold plasma (ACP) is a potential alternate to customary approaches for non-thermal decontamination of foods. In this study, the efficacy of a dielectric barrier discharge ACP system against ( Typhimurium, ATCC 13311; Choleraesuis, ATCC 10708) and (ATCC 25922, ATCC 11775) was explored. For each bacteria, a two-strain mixture at 8 log CFU/ml was spot inoculated on the surface of Golden Delicious apples, air dried, and exposed to ACP at a fixed distance of 35 mm, input power of 200 W for 30, 60, 120, 180, and 240 s. Bacterial inactivation was achieved in all treatment times with highest reduction of 5.3 log CFU/cm for and 5.5 log CFU/cm for . Our results showed that reductions were interrelated to exposure time and ranged from 1.3 to 5.3 and 0.6 to 5.5 log CFU/cm for and , respectively. and significantly decreased (>5.0 log) at 180 and 240 s as compared to 30, 60, and 120 s exposure. Microbial inactivation data was modeled by using Weibull distribution. These findings demonstrate the potential of ACP as a postharvest technology to effectively reduce pathogens on apples, with reference to and .
水果被人类病原体污染一直是新鲜农产品行业反复关注的问题。常压冷等离子体（ACP）是食品非热去污传统方法的一种潜在替代方法。在本研究中，探索了介质阻挡放电ACP系统对鼠伤寒沙门氏菌（ATCC 13311）、猪霍乱沙门氏菌（ATCC 10708）以及大肠杆菌（ATCC 25922、ATCC 11775）的杀菌效果。对于每种细菌，将浓度为8 log CFU/ml的两种菌株混合物点接种在金冠苹果表面，空气干燥后，在固定距离35 mm、输入功率200 W的条件下，分别暴露于ACP 30、60、120、180和240 s。在所有处理时间内均实现了细菌灭活，鼠伤寒沙门氏菌的最大减少量为5.3 log CFU/cm²，猪霍乱沙门氏菌为5.5 log CFU/cm²。我们的结果表明，减少量与暴露时间相关，鼠伤寒沙门氏菌和猪霍乱沙门氏菌的减少量分别为1.3至5.3 log CFU/cm²和0.6至5.5 log CFU/cm²。与暴露30、60和120 s相比，在暴露180和240 s时，鼠伤寒沙门氏菌和猪霍乱沙门氏菌显著减少（>5.0 log）。微生物灭活数据采用威布尔分布进行建模。这些发现证明了ACP作为一种采后技术在有效减少苹果上的病原体（针对鼠伤寒沙门氏菌和猪霍乱沙门氏菌）方面的潜力。
