Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing, China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China.
J Virol. 2024 Aug 20;98(8):e0061124. doi: 10.1128/jvi.00611-24. Epub 2024 Jul 30.
Porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus, is a serious threat to piglets and has zoonotic potential. Here, we aimed to further explore the role of aminopeptidase N (APN) as a receptor for PDCoV and test the inhibitory effect of a chimeric APN protein strategy on PDCoV infection. PK-15 cells and LLC-PK1 cells expressing chimeric APN were selected and infected with PDCoV. Viral replication was significantly decreased in these chimeric APN cells compared with that in control group cells. To further characterize the effect of the chimeric APN strategy on PDCoV infection , primary intestinal epithelial cells isolated from chimeric APN pigs were inoculated with PDCoV. Viral challenge of these cells led to decreased PDCoV infection. More importantly, virally challenged chimeric APN neonatal piglets displayed reduced viral load, significantly fewer microscopic lesions in the intestinal tissue, and no diarrhea. Taken together, these findings deepen our understanding of the mechanism of PDCoV infection and provide a valuable model for the production of disease-resistant animals.
Porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus, causes diarrhea in piglets and possesses the potential to infect humans. However, there are currently no effective measures for the prevention or control of PDCoV infection. Here, we have developed PK-15 cells, LLC-PK1 cells, and primary intestinal epithelial cells expressing chimeric APN, and viral challenge of these cells led to decreased PDCoV infection. Furthermore, virally challenged chimeric APN neonatal piglets displayed reduced viral load, significantly fewer microscopic lesions in the intestinal tissue, and no diarrhea. These data show that chimeric APN is a promising strategy to combat PDCoV infection.
猪德尔塔冠状病毒（PDCoV）是一种新兴的肠致病性冠状病毒，对仔猪构成严重威胁，且具有人畜共患病的潜力。在此，我们旨在进一步探索氨肽酶 N（APN）作为 PDCoV 受体的作用，并测试嵌合 APN 蛋白策略对 PDCoV 感染的抑制作用。选择表达嵌合 APN 的 PK-15 细胞和 LLC-PK1 细胞，并感染 PDCoV。与对照组细胞相比，这些嵌合 APN 细胞中的病毒复制明显减少。为了进一步表征嵌合 APN 策略对 PDCoV 感染的影响，用 PDCoV 感染从嵌合 APN 猪分离的原代肠上皮细胞。这些细胞的病毒攻击导致 PDCoV 感染减少。更重要的是，受病毒攻击的嵌合 APN 新生仔猪显示出较低的病毒载量、肠道组织中明显较少的微观病变且无腹泻。总之，这些发现加深了我们对 PDCoV 感染机制的理解，并为生产抗病动物提供了有价值的模型。
猪德尔塔冠状病毒（PDCoV）是一种新兴的肠致病性冠状病毒，会引起仔猪腹泻，并有可能感染人类。然而，目前尚无预防或控制 PDCoV 感染的有效措施。在此，我们已经开发出表达嵌合 APN 的 PK-15 细胞、LLC-PK1 细胞和原代肠上皮细胞，并且这些细胞的病毒攻击导致 PDCoV 感染减少。此外，受病毒攻击的嵌合 APN 新生仔猪显示出较低的病毒载量、肠道组织中明显较少的微观病变且无腹泻。这些数据表明，嵌合 APN 是对抗 PDCoV 感染的有前途的策略。
