Department of Pathology, College of Pharmacy, Hawler Medical University, Erbil, Iraq.
Department of Basic Sciences/Pathology, College of Medicine, Hawler Medical University, Erbil, Iraq.
J Infect Dev Ctries. 2024 Jul 29;18(7):1032-1040. doi: 10.3855/jidc.18150.
Cytokeratins (CKs) have been associated with precancerous and cancerous gastric lesions in patients with Helicobacter pylori-associated chronic gastritis, making them useful for diagnosing epithelial tumors.
A retrospective study was conducted utilizing 200 formalin-fixed paraffin-embedded gastric biopsy samples collected from the lesser curvature of the stomach. Samples from the control group, patients with H. pylori infection, and patients with H. pylori-associated gastritis, with complete and incomplete intestinal metaplasia (IM) were immunostained. Monoclonal antibodies were utilized to determine the expression of CK7, CK20, and Ki-67.
Patients infected with H. pylori had strong CK20 expression on the surface, and weak CK7 expression on the surface and deep glands; while non-specific chronic gastritis patients had weak focal CK7 expression and strong CK20 expression. The normal gastric mucosa of patients in the control group had relatively weak CK7 expression, restricted to a few cells in the neck and deep glands. CK20 showed diffuse strong reactivity on the surface. On the other hand, patients with complete IM showed a CK7 staining pattern that was either negative or weakly focal on the surface and crypts associated with diffuse surface CK20 and focal crypt staining corresponding to gastric type IM. The Ki67 proliferating index was low (≤ 15%) in H. pylori infected patients, high (> 30%) in patients with incomplete IM, and intermediate (16-30%) in patients with complete IM.
These results indicate a significant link between the expressions of CK7/CK20 and Ki67 in patients afflicted with H. pylori and IM.
细胞角蛋白（CKs）与幽门螺杆菌相关的慢性萎缩性胃炎患者的癌前和癌性胃病变有关，使其成为诊断上皮性肿瘤的有用工具。
对 200 例福尔马林固定石蜡包埋的胃活检样本进行回顾性研究，这些样本取自胃小弯。免疫染色了对照组、幽门螺杆菌感染患者和伴有不完全性和完全性肠上皮化生（IM）的幽门螺杆菌相关性胃炎患者的样本。使用单克隆抗体确定 CK7、CK20 和 Ki-67 的表达。
幽门螺杆菌感染患者的表面有强烈的 CK20 表达，表面和深部腺体有弱的 CK7 表达；而非特异性慢性胃炎患者有弱的局灶性 CK7 表达和强的 CK20 表达。对照组患者的正常胃黏膜 CK7 表达较弱，局限于颈部和深部腺体的少数细胞。CK20 在表面呈弥漫强反应性。另一方面，完全性 IM 患者的 CK7 染色模式表现为表面和隐窝的阴性或弱局灶性，伴有弥漫性表面 CK20 和对应于胃型 IM 的局灶性隐窝染色。Ki67 增殖指数在幽门螺杆菌感染患者中较低（≤15%），在不完全性 IM 患者中较高（>30%），在完全性 IM 患者中为中等（16-30%）。
这些结果表明，幽门螺杆菌感染和 IM 患者中 CK7/CK20 和 Ki67 的表达之间存在显著关联。
