Shannon J S, Lappin T R, Elder G E, Roberts G M, McGeown M G, Bridges J M
Clin Chim Acta. 1985 Dec 31;153(3):203-7. doi: 10.1016/0009-8981(85)90353-5.
We have measured plasma N-acetyl-beta-D-glucosaminidase (EC 3.2.1.30) and neuraminidase (EC 3.2.1.18) activities as markers of glycosidase activity and immunoreactive trypsin (EC 3.4.21.4) levels as a marker of proteolytic potential in the plasma of normal and uraemic subjects. The levels of all of these enzymes are significantly elevated in the plasma of uraemic subjects when compared to normal. We have postulated that the combined attack of glycosidases and proteases on erythropoietin will lead to fragmentation of this glycoprotein hormone with loss of activity. This may be a major contributory cause to the anaemia of chronic renal failure.
我们已测定正常人和尿毒症患者血浆中N-乙酰-β-D-氨基葡萄糖苷酶（EC 3.2.1.30）和神经氨酸酶（EC 3.2.1.18）的活性作为糖苷酶活性的标志物，以及免疫反应性胰蛋白酶（EC 3.4.21.4）水平作为血浆中蛋白水解潜能的标志物。与正常人相比，尿毒症患者血浆中所有这些酶的水平均显著升高。我们推测糖苷酶和蛋白酶对促红细胞生成素的联合攻击将导致这种糖蛋白激素的片段化并丧失活性。这可能是慢性肾衰竭贫血的一个主要促成原因。
