Department of Immunology, Xi'an Medical University, Xi'an, Shaanxi, China.
Affiliated Baoji Hospital of Xi'an Medical University, Xi'an, Shaanxi, China.
Mol Immunol. 2024 Sep;173:80-87. doi: 10.1016/j.molimm.2024.07.010. Epub 2024 Jul 29.
Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease that mainly affects the joints. Studies have shown that memory CD4 T cells play an important role in the pathogenesis of RA. This study investigated the expression and function of CD305 on human memory CD4 T cells and the effects of CD305 activating antibody on collagen-induced arthritis. The results showed that CD305 expression was significantly decreased on circulating memory CD4 T cells from patients with RA and its mean fluorescence intensity (MFI) was negatively correlated with DAS28. Moreover, CD305 inhibited the activation of memory CD4 T cells by down-regulating CD69 and CD25 and the production of IFN-γ, IL-4, and IL-17A induced by anti-CD3/CD28 antibodies. In addition, activation of CD305 inhibited the severity of disease in collagen-induced arthritis. In summary, CD305 reduction may mediate the excessive activation of memory CD4 T cells and participate in the development of RA. It can be used as a predictive marker of disease activity and has potential medicinal value in the treatment of RA.
类风湿关节炎（RA）是一种主要影响关节的慢性系统性自身免疫性疾病。研究表明，记忆 CD4 T 细胞在 RA 的发病机制中起重要作用。本研究探讨了人类记忆 CD4 T 细胞上 CD305 的表达和功能，以及 CD305 激活抗体对胶原诱导性关节炎的影响。结果表明，RA 患者循环记忆 CD4 T 细胞上 CD305 的表达显著降低，其平均荧光强度（MFI）与 DAS28 呈负相关。此外，CD305 通过下调 CD69 和 CD25 以及抑制抗 CD3/CD28 抗体诱导的 IFN-γ、IL-4 和 IL-17A 的产生来抑制记忆 CD4 T 细胞的活化。此外，CD305 的激活抑制了胶原诱导性关节炎的疾病严重程度。总之，CD305 的减少可能介导记忆 CD4 T 细胞的过度活化，并参与 RA 的发生发展。它可以作为疾病活动的预测标志物，在 RA 的治疗中具有潜在的药用价值。
