Zhao Zhengjia, Yan Weikai, Weng Xiaocheng
Department of Clinical Laboratory, Center for Gene Diagnosis, and Program of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University, China; College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan 430072, China.
Department of Clinical Laboratory, Center for Gene Diagnosis, and Program of Clinical Laboratory Medicine, Zhongnan Hospital of Wuhan University, China; College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan 430072, China.
Bioorg Med Chem. 2024 Sep 1;111:117861. doi: 10.1016/j.bmc.2024.117861. Epub 2024 Jul 28.
RNA modification identification is an emerging field in epigenetics due to its indispensable regulatory role in the cell life cycle. With advancements in identification methods, an increasing number of RNA modifications has been discovered, thereby driving the development of more efficient and accurate techniques for localizing modified RNAs and elucidating their functions. High-throughput sequencing approaches for modified RNA detection can be categorized into antibody-based, enzymatic-based, and chemical-labeling-based methods. Given the intrinsic chemical reactions involved in all biochemical processes, we provide a comprehensive review of recent advancements in artificial chemical labeling and transformations of ten distinct RNA modifications and their applications in sequencing. Our aim is to contribute to a deeper understanding of the mechanisms underlying these modifications. We focus on the chemical reactions associated with RNA modifications and briefly compare the advantages and disadvantages of detection methods based on these reactions. Additionally, we introduce several approaches that identify multiple modifications through chemical labeling. As the field of RNA modification research continues to expand, we anticipate that the techniques and insights presented in this review will serve as a valuable resource for future studies aimed at further elucidating the functional roles of RNA modifications in biological processes.
由于RNA修饰在细胞生命周期中具有不可或缺的调控作用，RNA修饰鉴定已成为表观遗传学领域的一个新兴方向。随着鉴定方法的进步，越来越多的RNA修饰被发现，从而推动了更高效、准确的定位修饰RNA及其功能的技术的发展。用于检测修饰RNA的高通量测序方法可分为基于抗体的方法、基于酶的方法和基于化学标记的方法。鉴于所有生化过程中都涉及内在化学反应，我们全面综述了十种不同RNA修饰的人工化学标记和转化及其在测序中的应用的最新进展。我们的目的是促进对这些修饰背后机制的更深入理解。我们重点关注与RNA修饰相关的化学反应，并简要比较基于这些反应的检测方法的优缺点。此外，我们介绍了几种通过化学标记鉴定多种修饰的方法。随着RNA修饰研究领域的不断扩展，我们预计本综述中介绍的技术和见解将为未来旨在进一步阐明RNA修饰在生物过程中的功能作用的研究提供宝贵资源。
