Vandivier Lee E, Gregory Brian D
University of Pennsylvania, Philadelphia, PA, United States; Cell and Molecular Biology Graduate Program, University of Pennsylvania, Philadelphia, PA, United States.
University of Pennsylvania, Philadelphia, PA, United States; Cell and Molecular Biology Graduate Program, University of Pennsylvania, Philadelphia, PA, United States; Genomics and Computational Biology Graduate Program, University of Pennsylvania, Philadelphia, PA, United States.
Enzymes. 2017;41:269-298. doi: 10.1016/bs.enz.2017.03.004. Epub 2017 Apr 14.
Ribonucleotides can be covalently modified with over 100 known chemical moieties, greatly expanding the potential coding and regulatory repertoire of RNA. Recent advances in applying high-throughput sequencing to the study of RNA modifications have generated transcriptome-wide modification maps and demonstrated that modifications are abundant features of multiple classes of RNAs, including messenger RNAs. In turn, new regulatory functions for RNA modifications have been elucidated. Here, we review both targeted and global methods for surveying RNA modification, with a focus on how transcriptome-wide methods have helped expand the understanding of modification-mediated regulation of the transcriptome.
核糖核苷酸可以与100多种已知化学基团进行共价修饰，极大地扩展了RNA潜在的编码和调控功能。将高通量测序应用于RNA修饰研究的最新进展已生成全转录组修饰图谱，并证明修饰是包括信使RNA在内的多类RNA的丰富特征。反过来，RNA修饰的新调控功能也已得到阐明。在这里，我们综述了用于检测RNA修饰的靶向方法和全局方法，重点关注全转录组方法如何有助于扩展对修饰介导的转录组调控的理解。
