Department for Small Animals, College of Veterinary Medicine, University of Leipzig, Leipzig, Germany.
Clinic of Small Animal Medicine, Ludwig Maximilian University of Munich, Munich, Germany.
N Z Vet J. 2024 Nov;72(6):317-322. doi: 10.1080/00480169.2024.2378696. Epub 2024 Jul 30.
To assess agreement of bacterial culture results from samples taken from nasal discharge, the nasal cavity and nasal biopsy from dogs and cats with nasal disease.
Nineteen dogs and 21 cats with different nasal diseases (chronic rhinitis, n = 30; neoplasia, n = 7; sinonasal aspergillosis, n = 3) were prospectively enrolled in the study. Nasal swabs were taken bilaterally from nasal discharge at the nares, the nasal cavity, and one nasal mucosal biopsy per side. All samples were subjected to aerobic bacterial culture. Kappa statistics were used to evaluate agreement for the most prevalent bacterial species between sampling sites.
A positive culture result for at least one bacterial species was detected in 80% of samples from nasal discharge/nares, 92% of nasal cavity samples, and 75% of biopsy samples. The mean agreement between the three sampling sites for positive negative culture results was never greater than moderate and the precision of the estimates of agreement varied widely.The most frequently isolated bacterial species in dogs were , spp. and spp. In cats, spp. and were the bacterial species cultured most frequently.For the most prevalent cultured species, spp., mean agreement between sites was never greater than fair and the precision again varied widely.
This study indicates that bacterial culture results in feline and canine nasal disease are site-specific and there was no evidence from this study for consistency between sites within a patient for many bacterial species. Consequently, if bacterial culture results from nasal swabs are used to guide therapeutic antimicrobial choice, different treatments may be selected depending on the site of culture. As a consequence, there is no evidence from this study that nasal bacterial cultures should be recommended as a routine diagnostic measure.
评估从患有鼻腔疾病的犬和猫的鼻腔分泌物、鼻腔和鼻腔活检样本中获取的细菌培养结果的一致性。
本前瞻性研究纳入了 19 只犬和 21 只患有不同鼻腔疾病（慢性鼻炎，n=30；肿瘤，n=7；鼻-鼻窦曲霉病，n=3）的猫和犬。双侧从鼻内取鼻腔分泌物拭子、鼻腔和每侧一个鼻腔黏膜活检样本。所有样本均进行需氧细菌培养。使用 Kappa 统计评估最常见细菌物种在采样部位之间的一致性。
在鼻腔分泌物/鼻腔的 80%、鼻腔样本的 92%和活检样本的 75%中至少检测到一种细菌培养阳性结果。三个采样部位之间阳性/阴性培养结果的平均一致性从未大于中度，并且一致性的估计精度差异很大。犬最常分离的细菌物种为 、 spp. 和 spp.。在猫中，最常培养的细菌物种为 spp. 和 。对于最常见的培养物种 spp.，部位之间的平均一致性从未大于一般，且精度再次差异很大。
本研究表明，在患有鼻腔疾病的猫和犬中，细菌培养结果是特定于部位的，在许多细菌物种方面，同一患者不同部位之间没有一致性的证据。因此，如果使用鼻腔拭子的细菌培养结果来指导治疗性抗菌药物选择，可能会根据培养部位选择不同的治疗方法。因此，本研究没有证据表明鼻腔细菌培养应作为常规诊断措施推荐。
