Department of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana, 141004, Punjab, India.
Electron Microscopy and Nanoscience Laboratory, Department of Soil Science, College of Agriculture, Punjab Agricultural University, Ludhiana, 141004, Punjab, India.
Mol Biol Rep. 2024 Jul 30;51(1):873. doi: 10.1007/s11033-024-09809-7.
Macrofungi commonly referred to as Mushrooms are distributed worldwide and well known for their nutritional, medicinal, and organoleptic properties. Strain improvement in mushrooms is lagging due to paucity of efficient genome modification techniques. Thus, for advanced developments in research and commercial or economical viability and benefit, CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9) emerged as an efficient genome editing tool. The higher efficiency and precision of the desired genetic modification(s) are the most valuable attributes of this recent technology. The present review comprehensively summarizes various conventional methods utilized for strain improvement in mushrooms including hybridization, protoplast fusion, and di-mon mating. Furthermore, the problems associated with these techniques have been discussed besides providing the potential recluses. The significance of CRISPR/Cas9 strategies employed for improvement in various mushroom genera has been deliberated, as these strategies will paves the way forward for obtaining improved strain and effective cultivation methods for enhancing the yield and quality of the fruit bodies.
大型真菌通常被称为蘑菇，分布广泛，以其营养、药用和感官特性而闻名。由于缺乏有效的基因组修饰技术，蘑菇的菌株改良进展缓慢。因此，为了在研究和商业或经济可行性和效益方面取得先进的发展，CRISPR/Cas9（成簇规律间隔短回文重复/CRISPR 相关核酸酶 9）作为一种有效的基因组编辑工具出现了。该新技术最有价值的属性是对所需遗传修饰（s）的更高效率和精度。本综述全面总结了用于蘑菇菌株改良的各种常规方法，包括杂交、原生质体融合和二倍体交配。此外，除了提供潜在的隐居地外，还讨论了与这些技术相关的问题。讨论了 CRISPR/Cas9 策略在各种蘑菇属中的应用对改良的重要性，因为这些策略将为获得改良的菌株和有效的栽培方法铺平道路，以提高子实体的产量和质量。
