Dhivya Gopi, Sridharan Kopula Sathyamoorthy, Nellepalli Sanjeeva Reddy, Kumar P Kennedy, Ramesh Arunagiri, Katta Divya
Department of Microbiology, Sri Ramachandra Medical College and Research Institute, SRIHER, Chennai, India.
Department of Laboratory Medicine, Sri Ramachandra Medical College and Research Institute, SRIHER, Chennai, India.
J Glob Infect Dis. 2024 Jun 26;16(2):68-72. doi: 10.4103/jgid.jgid_147_23. eCollection 2024 Apr-Jun.
Of the many sexually transmitted pathogens, is increasingly being associated with long-term sequelae such as infertility, apart from causing genital tract infections. Many inflammatory responses directed against chlamydial infection can cause tubal damage resulting in infertility. For example, chlamydial heat shock protein 60 (cHSP60) and cHSP10 along with humoral immune response. The aim of our study is to detect the presence of immunoglobulin G (IgG) antibodies against Major Outer Membrane Protein (MOMP), cHSP60, and cHSP10 among female infertility.
A total number of 230 female infertility patients attending the Outpatient Department of Reproductive Medicine, SRIHER, were included in the study. Detailed history documented in the proforma. Serological detection of . IgG antibody against MOMP, cHSP60, and cHSP10 antibody was done by enzyme-linked immunosorbent assay (ELISA).
. IgG antibody against MOMP was detected in 15 (6.5%) of 230 females. High seropositivity to cHSP60 antibodies was detected among females of tubal factor infertility (TFI). Our study showed that cHSP60 antibodies (3.4%) were more common than cHSP10 (2.6%).
Our study suggest cHSP60 or cHSP10 antibody detection by ELISA along with TFI is helpful for diagnosis and early institution of therapy. The accuracy of TFI prediction could be increased by the detection of anti-MOMP and cHSP60 over cHSP10 among secondary infertility than primary. The most probable reason for high seropositivity among secondary infertility patients may be due to repeated infection and chronicity because of longer active sexual life.
在众多性传播病原体中，除了引起生殖道感染外，它越来越多地与诸如不孕等长期后遗症相关联。许多针对衣原体感染的炎症反应可导致输卵管损伤，进而引起不孕。例如，衣原体热休克蛋白60（cHSP60）和cHSP10以及体液免疫反应。我们研究的目的是检测女性不孕症患者中针对主要外膜蛋白（MOMP）、cHSP60和cHSP10的免疫球蛋白G（IgG）抗体的存在情况。
本研究纳入了230名在SRIHER生殖医学门诊就诊的女性不孕症患者。在表格中记录详细病史。通过酶联免疫吸附测定（ELISA）对MOMP、cHSP60和cHSP10抗体进行血清学检测。
在230名女性中，有15名（6.5%）检测到针对MOMP的IgG抗体。在输卵管因素不孕症（TFI）女性中检测到对cHSP60抗体的高血清阳性率。我们的研究表明，cHSP60抗体（3.4%）比cHSP10（2.6%）更常见。
我们的研究表明，通过ELISA检测cHSP60或cHSP10抗体以及TFI有助于诊断和早期治疗。与原发性不孕症相比，在继发性不孕症中检测抗MOMP和cHSP60而非cHSP10可提高TFI预测的准确性。继发性不孕症患者血清阳性率高的最可能原因可能是由于活跃性生活时间较长导致反复感染和慢性感染。
