Department of Obstetrics and Gynecology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr., San Antonio, TX 78229,USA.
Am J Obstet Gynecol. 2010 Nov;203(5):494.e7-494.e14. doi: 10.1016/j.ajog.2010.06.005.
The objective of the study was to assess antibodies against Chlamydia trachomatis heat shock proteins (HSP) in patients with tubal factor infertility (TFI), infertility controls (IFC), and fertile controls (FC). HSPs assist organisms in surviving caustic environments such as heat.
Twenty-one TFI, 15 IFC, and 29 FC patients were enrolled after laparoscopic tubal assessment. The titers of antibodies against C trachomatis organisms and 14 chlamydial HSPs were compared among the 3 groups.
TFI patients developed significantly higher levels of antibodies against C trachomatis and specifically recognizing chlamydial HSP60 and caseinolytic protease (Clp) P, a subunit of the ATP-dependent Clp protease complex involved in the degradation of abnormal proteins.
In addition to confirming high titers of antibodies against C trachomatis organisms and HSP60 in TFI patients, we identified a novel link of TFI with anti-ClpP antibodies. These findings may provide useful information for developing a noninvasive screening test for TFI and constructing subunit anti-C trachomatis vaccines.
本研究旨在评估输卵管因素不孕（TFI）患者、不孕对照组（IFC）和正常生育对照组（FC）中针对沙眼衣原体热休克蛋白（HSP）的抗体。HSP 可帮助生物体在如热等腐蚀性环境中存活。
在腹腔镜输卵管评估后，共纳入 21 例 TFI、15 例 IFC 和 29 例 FC 患者。比较了 3 组患者针对沙眼衣原体生物体和 14 种沙眼衣原体 HSP 的抗体滴度。
TFI 患者针对沙眼衣原体的抗体水平以及针对 HSP60 和蛋白酶体 ClpP（ATP 依赖性 Clp 蛋白酶复合物的一个亚基，参与异常蛋白的降解）的特异性抗体水平显著升高。
除了在 TFI 患者中证实了针对沙眼衣原体生物体和 HSP60 的高滴度抗体外，我们还发现 TFI 与抗 ClpP 抗体有关。这些发现可能为开发 TFI 的非侵入性筛查试验和构建亚单位抗沙眼衣原体疫苗提供有用信息。
