Balkrishna Acharya, Joshi Monali, Kabdwal Manisha, Tomer Meenu, Lochab Savita, Varshney Anurag
Drug Discovery and Development Division, Patanjali Research Foundation, Haridwar, Uttarakhand, India.
Department of Allied and Applied Sciences, University of Patanjali, Haridwar, Uttarakhand, India.
Front Microbiol. 2024 Jul 16;15:1429027. doi: 10.3389/fmicb.2024.1429027. eCollection 2024.
The formidable survival mechanisms employed by (), combined with the low bioavailability of anti-tubercular drugs and their associated hepatotoxicity, worsen tuberculosis management. Traditional medicinal plants offer potential solutions to these challenges. This study focuses on exploring the anti-tubercular potential of against , .
HPTLC and UHPLC phytochemically characterized the hydro-methanolic extract of (SVE). SVE curtails the growth and viability of under normal and stress conditions. The compromised cell wall integrity of with SVE is depicted through scanning electron microscopy (SEM) while EtBr permeability assays and TLC-based comparative changes in lipids extraction addressed the integrity of the cell wall. Furthermore, SVE augmented the susceptibility of towards Isoniazid (INH) through enhanced bioavailability. Adjunct treatment of SVE with INH demonstrated a markedly reduced survival of the intracellular bacilli. The study also uncovered the hepatoprotective potential of SVE in HepG2 cells.
This research paves the way for deeper exploration into the potential of against virulent strains, emphasizing over the significance of traditional medicinal plants in tuberculosis treatment. Collectively, the findings suggest SVE as a potent candidate for independent or adjunct anti-tubercular therapy.
（）所采用的强大生存机制，再加上抗结核药物的低生物利用度及其相关的肝毒性，使结核病的治疗变得更加困难。传统药用植物为这些挑战提供了潜在的解决方案。本研究重点探索（植物名称未给出）对（结核分枝杆菌菌株名称未给出）的抗结核潜力。
采用高效薄层色谱（HPTLC）和超高效液相色谱（UHPLC）对（植物名称未给出）的水甲醇提取物（SVE）进行了植物化学表征。SVE在正常和（胁迫条件未明确给出）胁迫条件下均能抑制（结核分枝杆菌菌株名称未给出）的生长和活力。通过扫描电子显微镜（SEM）描绘了SVE处理后（结核分枝杆菌菌株名称未给出）细胞壁完整性受损的情况，而溴化乙锭渗透性测定和基于薄层色谱的脂质提取比较变化则研究了细胞壁的完整性。此外，SVE通过提高生物利用度增强了（结核分枝杆菌菌株名称未给出）对异烟肼（INH）的敏感性。SVE与INH联合治疗显示细胞内杆菌的存活率显著降低。该研究还发现了SVE在HepG2细胞中的肝保护潜力。
本研究为深入探索（植物名称未给出）对强毒（结核分枝杆菌菌株名称未给出）菌株的潜力铺平了道路，强调了传统药用植物在结核病治疗中的重要性。总的来说，研究结果表明SVE是独立或辅助抗结核治疗的有力候选药物。
