Bähler M, Moser H, Eppenberger H M, Wallimann T
Dev Biol. 1985 Dec;112(2):345-52. doi: 10.1016/0012-1606(85)90405-1.
The expression of cardiac and white skeletal C-protein isoforms was analyzed in developing chicken embryos and in primary skeletal muscle cell cultures by immunoblot and immunofluorescence staining using polyclonal antibodies specific for both of the two different proteins. In the embryo, cardiac C-protein was detected in the developing heart from very early stages through adulthood. In skeletal muscle, cardiac C-protein is shown to be transiently expressed between Days 3 and 15 during development. In contrast, the expression of white skeletal C-protein is gradual and progressive starting approximately from Day 15 on in development. In primary cell cultures of skeletal muscle, however, cardiac C-protein remained expressed throughout prolonged culture time, this in conjunction with white skeletal C-protein. Thus the down regulation of cardiac C-protein and the transition from cardiac C-protein to adult skeletal (white) C-protein which was observed during skeletal muscle development in vivo, does not seem to go to completion in the in vitro system.
利用针对两种不同蛋白质的多克隆抗体，通过免疫印迹和免疫荧光染色，分析了发育中的鸡胚以及原代骨骼肌细胞培养物中心脏型和白色骨骼肌型C蛋白亚型的表达情况。在胚胎中，从早期到成年期的发育心脏中均能检测到心脏型C蛋白。在骨骼肌中，心脏型C蛋白在发育的第3天至第15天之间短暂表达。相比之下，白色骨骼肌型C蛋白的表达从发育过程中大约第15天开始逐渐递增。然而，在骨骼肌原代细胞培养物中，心脏型C蛋白在整个延长的培养时间内持续表达，与白色骨骼肌型C蛋白同时存在。因此，在体内骨骼肌发育过程中观察到的心脏型C蛋白的下调以及从心脏型C蛋白向成年骨骼肌（白色）C蛋白的转变，在体外系统中似乎并未完全完成。
