Golcuk Oral and Dental Health Center, Golcuk, Kocaeli, Turkey.
Department of Endodontics, Faculty of Dentistry, Kirikkale University, Kirikkale, Turkey.
Niger J Clin Pract. 2024 Jul 1;27(7):897-904. doi: 10.4103/njcp.njcp_126_24. Epub 2024 Jul 27.
Regenerative endodontics involves the use of various root canal medicaments and scaffolds, which may cause crown discoloration.
This study aimed to investigate the combined crown discoloration of scaffolds [platelet-rich fibrin (PRF) and blood clot] applied after administration of different medicaments [modified triple antibiotic paste including doxycycline (mTAPd), modified double antibiotic paste (mDAP), calcium hydroxide (CH), and propolis].
In total, 100 human mandibular premolar teeth were selected and prepared. The teeth were apically resected to simulate immature teeth. The positive and negative control groups (n = 10) consisted solely of blood-only and serum-only samples. The remaining 80 teeth were used for the experimental groups with four different medicaments. Three weeks later, either blood or PRF was applied as a scaffold after removing the medicaments (n = 10). Color changes were assessed before medication placement and at the end of the first, second, and third weeks, as well as on days 0, 1, 30, 60, and 90 after scaffold application. Analysis was carried out using repeated measures of variance, Friedman, one-way ANOVA, Kruskal-Wallis, the dependent paired t-test, and Wilcoxon test.
Statistical significance was determined at P = 0.05. All groups including blood and the group including propolis and PRF combination, resulted in a significant increase in discoloration (P < 0.05) and discoloration exceeding clinically acceptable thresholds.
CH and the modified versions of TAP (mTAPd) and DAP (mDAP) demonstrated an acceptable level of discoloration when used with a combination of PRF at day 90.
再生牙本质内含有各种根管药物和支架，这可能会导致牙冠变色。
本研究旨在探讨不同药物[含多西环素的改良三重抗生素糊剂（mTAPd）、改良双抗生素糊剂（mDAP）、氢氧化钙（CH）和蜂胶]处理后应用的支架[富血小板纤维蛋白（PRF）和血凝块]联合牙冠变色情况。
共选取 100 颗人下颌前磨牙，制备并根尖切除以模拟未成熟恒牙。阳性和阴性对照组（n = 10）仅为血样和血清样。剩余 80 颗牙用于实验组，每组分别使用 4 种不同的药物。3 周后，在去除药物后分别应用血液或 PRF 作为支架（n = 10）。在放置药物前、第 1、2、3 周末以及支架放置后第 0、1、30、60 和 90 天评估颜色变化。采用重复测量方差、Friedman、单因素方差分析、Kruskal-Wallis、配对 t 检验和 Wilcoxon 检验进行分析。
P = 0.05 时差异有统计学意义。所有包括血液的组和包括蜂胶与 PRF 组合的组，变色显著增加（P < 0.05）且变色超过临床可接受阈值。
CH 和改良的 TAP（mTAPd）和 DAP（mDAP）与 PRF 联合应用时，在第 90 天显示出可接受的变色水平。
