Japan Collection of Microorganisms RIKEN BioResource Research Center, 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
Department of Clinical Plant Science, Faculty of Bioscience, Hosei University, Koganei, Tokyo, 184-8584, Japan.
Antonie Van Leeuwenhoek. 2024 Jul 31;117(1):109. doi: 10.1007/s10482-024-01990-w.
The genetic variety and habitats of Camptophora species, generally known as black yeast, have not been clarified. In this study, we re-evaluated Camptophora based on morphological observations and phylogenetic analyses. Because prior investigations on Camptophora only included a few strains/specimens, 24 Camptophora-related strains were newly obtained from 13 leaf samples of various plant species to redefine the genetic and species concepts of Camptophora. Their molecular phylogenetic relationships were examined using small subunit nuclear ribosomal DNA (nSSU, 18S rDNA), the internal transcribed spacer (ITS) rDNA operon, the large subunit nuclear ribosomal DNA (LSU, 28S rDNA), β-tubulin, the second largest subunit of RNA polymerase II (rpb2), and mitochondrial small subunit DNA (mtSSU). Single- and multi-locus analyses using nSSU-ITS-LSU-rpb2-mtSSU revealed a robust phylogenetic relationship among Camptophora species within Chaetothyriaceae. Camptophora species could be distinguished from other chaetothyriaceous genera by their snake-shaped conidia with microcyclic conidiation and loosely interwoven mycelial masses. Based on the results of phylogenetic analyses, two undescribed lineages were recognized, and Ca. schimae was excluded from the genus. ITS sequence comparison with environmental DNA sequences revealed that the distribution of the genus is restricted to the Asia-Pacific region. Camptophora has been isolated or detected from abrupt sources, and this was attributed to its microcycle. The mechanisms driving genetic diversity within species are discussed with respect to their phyllosphere habitats.
黑酵母属（Camptophora）物种的遗传多样性和栖息地尚未得到阐明。在本研究中，我们基于形态学观察和系统发育分析对黑酵母属进行了重新评估。由于先前对黑酵母属的研究仅包含少数菌株/标本，我们从 13 种不同植物叶片样本中获得了 24 株新的黑酵母属相关菌株，以重新定义黑酵母属的遗传和物种概念。我们使用小亚基核核糖体 DNA（nSSU，18S rDNA）、内部转录间隔区（ITS）rDNA 操纵子、大亚基核核糖体 DNA（LSU，28S rDNA）、β-微管蛋白、RNA 聚合酶 II 第二大亚基（rpb2）和线粒体小亚基 DNA（mtSSU）对这些菌株的分子系统发育关系进行了检测。使用 nSSU-ITS-LSU-rpb2-mtSSU 进行的单基因和多基因分析显示，在 chaetothyriaceae 内黑酵母属物种之间存在稳健的系统发育关系。黑酵母属物种可以通过其具有微循环的蛇形分生孢子和松散交织的菌丝体来与其他 chaetothyriaceous 属区分开来。基于系统发育分析的结果，识别出两个未描述的谱系，并将 Ca. schimae 排除在属之外。与环境 DNA 序列的 ITS 序列比较表明，该属的分布仅限于亚太地区。黑酵母属已从突发源中被分离或检测到，这归因于其微循环。关于其叶际生境，讨论了种内遗传多样性的驱动机制。
