School of Agroforestry and Medicine, The Open University of China, Beijing 100039, China.
Institute of Animal Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
J Vet Sci. 2024 Jul;25(4):e54. doi: 10.4142/jvs.24069.
As one of the main etiologic agents of infectious diseases in pigs, pseudorabies virus (PRV) infections have caused enormous economic losses worldwide. EP0, one of the PRV early proteins (EP) plays a vital role in PRV infections, but the mechanisms are unclear.
This study examined the function of EP0 to provide a direction for its in-depth analysis.
In this study, the EP0-deleted PRV mutant was obtained, and Tandem Mass Tag-based proteomic analysis was used to screen the differentially expressed proteins (DEPs) quantitatively in EP0-deleted PRV- or wild-type PRV-infected porcine kidney 15 cells.
This study identified 7,391 DEPs, including 120 and 21 up-regulated and down-regulated DEPs, respectively. Western blot analysis confirmed the changes in the expression of the selected proteins, such as speckled protein 100. Comprehensive analysis revealed 141 DEPs involved in various biological processes and molecular functions, such as transcription regulator activity, biological regulation, and localization.
These results holistically outlined the functions of EP0 during a PRV infection and might provide a direction for more detailed function studies of EP0 and the stimulation of lytic PRV infections.
作为猪传染性疾病的主要病原之一，伪狂犬病病毒（PRV）感染在全球范围内造成了巨大的经济损失。EP0 是 PRV 的主要早期蛋白（EP）之一，在 PRV 感染中起着至关重要的作用，但具体机制尚不清楚。
本研究旨在探讨 EP0 的功能，为其深入分析提供方向。
本研究获得了缺失 EP0 的 PRV 突变株，并采用串联质量标签（Tandem Mass Tag）基于蛋白质组学分析定量筛选 EP0 缺失的 PRV 或野生型 PRV 感染猪肾 15 细胞中的差异表达蛋白（DEPs）。
本研究共鉴定到 7391 个 DEPs，其中分别有 120 个和 21 个上调和下调的 DEPs。Western blot 分析验证了所选蛋白表达的变化，如斑点蛋白 100。综合分析揭示了 141 个 DEPs 参与了各种生物学过程和分子功能，如转录调控活性、生物调节和定位。
这些结果全面概述了 EP0 在 PRV 感染过程中的功能，可能为更详细的 EP0 功能研究和刺激裂解型 PRV 感染提供方向。
