Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
Viruses. 2022 Apr 4;14(4):755. doi: 10.3390/v14040755.
Suid herpesvirus 1 (SuHV-1), known as pseudorabies virus (PRV), is one of the most devastating swine pathogens in China, particularly the sudden occurrence of PRV variants in 2011. The higher pathogenicity and cross-species transmission potential of the newly emerged variants caused not only colossal economic losses, but also threatened public health. To uncover the underlying pathogenesis of PRV variants, Tandem Mass Tag (TMT)-based proteomic analysis was performed to quantitatively screen the differentially expressed cellular proteins in PRV-infected Vero cells. A total of 7072 proteins were identified and 960 proteins were significantly regulated: specifically 89 upregulated and 871 downregulated. To make it more credible, the expression of XRCC5 and XRCC6 was verified by western blot and RT-qPCR, and the results dovetailed with the proteomic data. The differentially expressed proteins were involved in various biological processes and signaling pathways, such as chaperonin-containing T-complex, NIK/NF-κB signaling pathway, DNA damage response, and negative regulation of G2/M transition of mitotic cell cycle. Taken together, our data holistically outline the interactions between PRV and host cells, and our results may shed light on the pathogenesis of PRV variants and provide clues for pseudorabies prevention.
猪疱疹病毒 1（SuHV-1），又称伪狂犬病病毒（PRV），是中国危害最严重的猪病病原体之一，特别是 2011 年 PRV 变异株的突然出现。新出现的变异株具有更高的致病性和跨种传播潜力，不仅造成了巨大的经济损失，还威胁到了公共卫生。为了揭示 PRV 变异株的潜在发病机制，我们采用串联质量标签（TMT）-基于蛋白质组学分析来定量筛选 PRV 感染 Vero 细胞中的差异表达细胞蛋白。共鉴定出 7072 种蛋白质，其中 960 种蛋白质显著调节：具体来说，有 89 种上调，871 种下调。为了使其更可信，我们通过 Western blot 和 RT-qPCR 验证了 XRCC5 和 XRCC6 的表达，结果与蛋白质组学数据一致。差异表达蛋白参与了各种生物过程和信号通路，如伴侣蛋白含 T 复合物、NIK/NF-κB 信号通路、DNA 损伤反应和有丝分裂细胞周期 G2/M 期的负调控。总之，我们的数据全面概述了 PRV 与宿主细胞之间的相互作用，我们的结果可能揭示了 PRV 变异株的发病机制，并为伪狂犬病的预防提供了线索。
