Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China.
J Agric Food Chem. 2024 Aug 14;72(32):17868-17879. doi: 10.1021/acs.jafc.4c03896. Epub 2024 Jul 31.
is a critical global quarantine pest. Recently, its distribution has been extended to colder and higher-latitude regions. The adaptation to low temperatures is vital for the successful colonization of insects in new environments. However, the metabolic pathways of larvae under cold stress remain undefined. This study analyzed the larval hemolymph under different low-temperature treatments using LC-MS/MS. The results showed that differential metabolites associated with sugar and lipid metabolism are pivotal in the larval chill coma process. Under low-temperature treatments, the glycerol content increased significantly compared with the control group. Cold stress significantly induced the expression of and . After undergoing RNAi treatment for 48 h, larvae exposed to -20 °C for 1 h showed reduced recovery when injected with ds- and ds- compared to the control group, indicating that glycerol biosynthesis plays a role in the low-temperature adaptation of larvae. Our results provide a theoretical basis for clarifying the molecular mechanism of larvae in response to environmental stresses.
是一种重要的全球检疫害虫。最近，它的分布范围已经扩展到了更寒冷和高纬度的地区。昆虫在新环境中的成功定殖对低温适应至关重要。然而，幼虫在低温胁迫下的代谢途径仍不清楚。本研究使用 LC-MS/MS 分析了不同低温处理下幼虫的血淋巴。结果表明，与糖和脂质代谢相关的差异代谢物在幼虫冷昏迷过程中起关键作用。在低温处理下，与对照组相比，甘油含量显著增加。冷胁迫显著诱导 和 的表达。经过 48 h 的 RNAi 处理后，与对照组相比，在 -20°C 下处理 1 小时后再注射 ds-和 ds-的幼虫恢复能力降低，表明甘油生物合成在 幼虫的低温适应中起作用。我们的研究结果为阐明 幼虫应对环境胁迫的分子机制提供了理论依据。
