Glycerol Metabolism is Important for the Low-Temperature Adaptation of a Global Quarantine Pest Larvae.

is a critical global quarantine pest. Recently, its distribution has been extended to colder and higher-latitude regions. The adaptation to low temperatures is vital for the successful colonization of insects in new environments. However, the metabolic pathways of larvae under cold stress remain undefined. This study analyzed the larval hemolymph under different low-temperature treatments using LC-MS/MS. The results showed that differential metabolites associated with sugar and lipid metabolism are pivotal in the larval chill coma process. Under low-temperature treatments, the glycerol content increased significantly compared with the control group. Cold stress significantly induced the expression of and . After undergoing RNAi treatment for 48 h, larvae exposed to -20 °C for 1 h showed reduced recovery when injected with ds- and ds- compared to the control group, indicating that glycerol biosynthesis plays a role in the low-temperature adaptation of larvae. Our results provide a theoretical basis for clarifying the molecular mechanism of larvae in response to environmental stresses.