Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China.
Int J Mol Sci. 2022 Oct 8;23(19):11958. doi: 10.3390/ijms231911958.
() is an important global quarantine pest due to its highly destructive impacts on forests. It is widely distributed in many countries in Asia, Europe, and North America. The survival of larvae has been facilitated by its high adaptability to low temperature. When insects are subjected to temperature stress, heat shock proteins (Hsps) limit cell damage and improve cell tolerance via their protein folding, localization, and degradation activities. However, the temperature adaptation mechanisms of Hsps remain unclear. In this study, four genes, , , , and , were cloned. Sequence analysis showed that all four Hsps had specific conserved domains. Phylogenetic analysis revealed that Hsps from different subfamilies were evolutionarily conserved, and that AglaHsps were highly similar to those of Coleoptera species. Protein expression vectors (pET30a-) were constructed and used to express AglaHsps in , where all four proteins were expressed in inclusion bodies. Western blot analysis showed that AglaHsps were expressed at a range of temperatures, from -10 °C to 25 °C. AglaHsp82.09 and AglaHsp89.76 showed high expressions with treatment at 0 °C. Our results will facilitate clarification of the molecular mechanisms underlying responses to environmental stress.
松褐天牛是一种重要的全球检疫害虫，因其对森林具有高度破坏性的影响而受到广泛关注。它广泛分布在亚洲、欧洲和北美洲的许多国家。幼虫的生存得益于其对低温的高度适应性。当昆虫受到温度胁迫时，热休克蛋白（Hsps）通过其蛋白质折叠、定位和降解活性来限制细胞损伤并提高细胞耐受性。然而，松褐天牛 Hsps 的温度适应机制尚不清楚。本研究克隆了四个 基因，分别是 、 、 、和 。序列分析表明，所有四个 Hsps 都具有特定的保守结构域。系统发育分析表明，来自不同亚家族的 Hsps 在进化上是保守的，并且 AglaHsps 与鞘翅目物种的 Hsps 高度相似。构建了蛋白表达载体（pET30a-），并在 中表达 AglaHsps，其中所有四种蛋白均以包涵体形式表达。Western blot 分析表明，AglaHsps 在-10°C 至 25°C 的温度范围内表达。AglaHsp82.09 和 AglaHsp89.76 在 0°C 处理时表现出较高的表达水平。我们的研究结果将有助于阐明松褐天牛对环境胁迫的分子机制。
