Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang, 550025, China; Institute of Animal Nutrition and Feed Science, Guizhou University, Guiyang, 550025, China.
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University, Guiyang, 550025, China; Institute of Animal Nutrition and Feed Science, Guizhou University, Guiyang, 550025, China.
Biochem Biophys Res Commun. 2024 Nov 19;734:150460. doi: 10.1016/j.bbrc.2024.150460. Epub 2024 Jul 27.
Dihydroquercetin (DHQ), also known as Taxifolin (TA), is a flavanonol with various biological activities, such as anticancer, anti-inflammatory, and antioxidative properties. It has been found to effectively increase the viability of porcine intestinal epithelial cells (IPEC-J2). However, the precise mechanism by which DHQ increases the proliferation of IPEC-J2 cells is not entirely understood. This study aimed to explore the potential pathways through which DHQ encourages the proliferation of IPEC-J2 cells. The findings indicated that DHQ significantly improved the protein expression of tight junction proteins (ZO-1, Occludin, and Claudin1) and a molecular biomarker of proliferation (PCNA) in IPEC-J2 cells. Furthermore, DHQ was found to increase the Wnt/β-catenin pathway-associated β-catenin, c-Myc, and cyclin D1 mRNA expression, and promote the protein expression of β-catenin and TCF4. To confirm the involvement of the Wnt/β-catenin signaling pathway in the DHQ-promoted proliferation of IPEC-J2 cells, the inhibitor LF3, which targets β-catenin/TCF4 interaction, was used. It was found that LF3 inhibited the protein expressions upregulated by DHQ and blocked the promotion of cell proliferation. These results indicate that DHQ positively regulates IPEC-J2 cell proliferation through the Wnt/β-catenin pathway, providing constructive insights into the role of DHQ in regulating intestine development.
二氢槲皮素（DHQ），又称 Taxifolin（TA），是一种具有多种生物活性的黄烷醇，如抗癌、抗炎和抗氧化特性。它已被发现能有效地提高猪肠上皮细胞（IPEC-J2）的活力。然而，DHQ 促进 IPEC-J2 细胞增殖的确切机制尚不完全清楚。本研究旨在探讨 DHQ 促进 IPEC-J2 细胞增殖的潜在途径。研究结果表明，DHQ 显著改善了 IPEC-J2 细胞中紧密连接蛋白（ZO-1、Occludin 和 Claudin1）和增殖的分子生物标志物（PCNA）的蛋白表达。此外，DHQ 被发现增加了 Wnt/β-连环蛋白通路相关的β-连环蛋白、c-Myc 和细胞周期蛋白 D1mRNA 的表达，并促进了β-连环蛋白和 TCF4 的蛋白表达。为了证实 Wnt/β-连环蛋白信号通路在 DHQ 促进 IPEC-J2 细胞增殖中的作用，使用了靶向β-连环蛋白/TCF4 相互作用的抑制剂 LF3。结果发现，LF3 抑制了 DHQ 上调的蛋白表达，并阻断了细胞增殖的促进作用。这些结果表明，DHQ 通过 Wnt/β-连环蛋白通路正向调节 IPEC-J2 细胞增殖，为 DHQ 在调节肠道发育中的作用提供了有益的见解。
