School of Materials Science and Engineering, University of Jinan, Jinan, 250022, Shandong, China.
State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, Shandong, China.
Talanta. 2024 Nov 1;279:126605. doi: 10.1016/j.talanta.2024.126605. Epub 2024 Jul 24.
Intracellular lipid droplets (LDs) are important organelles regulating intracellular redox processes. Endogenous bisulfite/sulfite (HSO/SO) is one of the metabolites of thiol metabolism. The variation in HSO/SO content around LDs is closely related to cellular homeostasis. However, there is currently no effective method to visualize and quantify the dynamic changes in HSO/SO content around LDs. In this work, a fluorescent probe MC-BEN utilizing a triphenylamine basic framework was developed to selectively recognize HSO/SO via a nucleophilic addition reaction. The probe exhibits excellent anti-interference capability, short response time, outstanding photostability, and a low fluorescence detection limit (6.1 μM) for HSO/SO recognition. More interesting, there is a trend of accelerated contact between LDs and lysosomes after MC-BEN targeting LDs and reacting with endogenous/exogenous HSO/SO, which may provide new ideas for the study of intracellular lysosomal lipophagy.
细胞内脂滴 (LDs) 是调节细胞内氧化还原过程的重要细胞器。内源性亚硫酸氢盐/亚硫酸盐 (HSO/SO) 是硫醇代谢的一种代谢物。LDs 周围 HSO/SO 含量的变化与细胞内稳态密切相关。然而，目前还没有有效的方法来可视化和定量检测 LDs 周围 HSO/SO 含量的动态变化。在这项工作中，开发了一种基于三苯胺碱性骨架的荧光探针 MC-BEN，通过亲核加成反应选择性识别 HSO/SO。该探针具有出色的抗干扰能力、短的响应时间、出色的光稳定性和对 HSO/SO 识别的低荧光检测限（6.1 μM）。更有趣的是，MC-BEN 靶向 LDs 并与内源性/外源性 HSO/SO 反应后，LDs 与溶酶体之间的接触呈现加速趋势，这可能为研究细胞内溶酶体脂解提供新的思路。
