Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China; Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, School of Basic Medical Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China; Intensive Care Unit, Shenzhen Second People's Hospital, the First Affiliated Hospital of Shenzhen University, Shenzhen 518031, China.
Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
Biomed Pharmacother. 2024 Sep;178:117224. doi: 10.1016/j.biopha.2024.117224. Epub 2024 Jul 30.
Ventilator-associated pneumonia (VAP) is a common healthcare-acquired infection often arising during artificial ventilation using endotracheal intubation (ETT), which offers a platform for bacterial colonization and biofilm development. In particular, the effects of prolonged COVID-19 on the respiratory system. Herein, we developed an antimicrobial coating (FK-MEM@CMCO-CS) capable of visualizing pH changes based on bacterial infection and releasing meropenem (MEM) and FK13-a1 in a controlled manner. Using a simple dip-coating process with controlled loading, chitosan was cross-linked with sodium carboxymethyl cellulose oxidation (CMCO) and coated onto PVC-based ETT to form a hydrogel coating. Subsequently, the coated segments were immersed in an indicator solution containing bromothymol blue (BTB), MEM, and FK13-a1 to fabricate the FK-MEM@CMCO-CS coating. In vitro studies have shown that MEM and FK13-a1 can be released from coatings in a pH-responsive manner. Moreover, anti-biofilm and antibacterial adhesion results showed that FK-MEM@CMCO-CS coating significantly inhibited biofilm formation and prevented their colonization of the coating surface. In the VAP rat model, the coating inhibited bacterial growth, reduced lung inflammation, and had good biocompatibility. The coating can be applied to the entire ETT and has the potential for industrial production.
呼吸机相关性肺炎（VAP）是一种常见的医源性感染，通常发生在使用气管插管（ETT）进行人工通气期间，这为细菌定植和生物膜形成提供了平台。特别是，COVID-19 对呼吸系统的长期影响。在此，我们开发了一种抗菌涂层（FK-MEM@CMCO-CS），能够根据细菌感染可视化 pH 值变化，并以受控方式释放美罗培南（MEM）和 FK13-a1。通过使用具有受控负载的简单浸涂工艺，壳聚糖与氧化羧甲基纤维素钠（CMCO）交联，并涂覆到基于 PVC 的 ETT 上形成水凝胶涂层。随后，将涂覆的部分浸入含有溴百里酚蓝（BTB）、MEM 和 FK13-a1 的指示剂溶液中，以制备 FK-MEM@CMCO-CS 涂层。体外研究表明，MEM 和 FK13-a1 可以通过 pH 响应从涂层中释放。此外，抗生物膜和抗菌粘附结果表明，FK-MEM@CMCO-CS 涂层显著抑制生物膜形成并防止其在涂层表面定植。在 VAP 大鼠模型中，该涂层抑制了细菌生长，减轻了肺部炎症，具有良好的生物相容性。该涂层可应用于整个 ETT，具有工业化生产的潜力。
