College of Environment and Resources, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental and Ecological Health, Xiangtan University, Xiangtan 411105, China; Hunan Provincial University Key Laboratory for Environmental Behavior and Control Principle of New Pollutants, Xiangtan University, Xiangtan 411105, China.
Shanghai Academy of Environmental Sciences, Shanghai 200233, China.
Environ Int. 2024 Aug;190:108915. doi: 10.1016/j.envint.2024.108915. Epub 2024 Jul 28.
Pathogenic microorganisms with antibiotic resistance genes (ARGs) pose a serious threat to public health and soil ecology. Although new drugs and available antibacterial materials can kill ARG carriers but accidentally kill beneficial microorganisms. Therefore, the rapid enrichment and separation of ARGs and their carriers from soil is becoming an important strategy for controlling the diffusion of ARGs. Hydroxamate siderophore (HDS) has gained widespread attentions for its involvement in trace element transfer among microorganisms in the soil environment, we thus explored an in-situ trapping-enrichment method for ARGs and their carriers via a small molecular HDS secreted by Pseudomonas fluorescens HMP01. In this study, we demonstrate that HDS significantly in-situ traps and enriches certain ARGs, including chloramphenicol, MLS, rifamycin, and tetracycline resistance genes in the soil environment. The enrichment efficiencies were 1473-fold, 38-fold, 17-fold, and 5-fold, respectively, higher than those in the control group. Specifically, the primary enriched ARGs were rpoB, mphL, catB2, and tetA(60), and Bacillus, Rhizobium, Rossellomorea, and Agrobacterium were hosts for these ARGs. This enrichment was caused by the upregulation of chemotaxis genes (e.g., cheW, cheC, and cheD) and rapid biofilm formation within the enriched bacterial population. Notably, representative ARGs such as cat, macB, and rpoB were significantly reduced by 36%, 85.7%, and 72%, respectively, in the paddy soil after HDS enrichment. Our research sheds light on the potential application of siderophore as a rapping agent for the eco-friendly reduction of ARGs and their carriers in soil environments.
具有抗生素耐药基因 (ARGs) 的致病微生物对公众健康和土壤生态构成严重威胁。尽管新的药物和现有的抗菌材料可以杀死 ARG 携带者，但也会意外杀死有益的微生物。因此，从土壤中快速富集和分离 ARGs 及其载体正成为控制 ARGs 扩散的重要策略。羟肟酸类铁载体 (HDS) 因其参与土壤环境中微生物之间的微量元素转移而受到广泛关注，因此我们探索了一种通过荧光假单胞菌 HMP01 分泌的小分子 HDS 原位捕获和富集土壤中 ARGs 及其载体的方法。在本研究中，我们证明 HDS 可显著原位捕获和富集土壤环境中的某些 ARGs，包括氯霉素、MLS、利福平和四环素耐药基因。与对照组相比，富集效率分别提高了 1473 倍、38 倍、17 倍和 5 倍。具体而言，主要富集的 ARGs 是 rpoB、mphL、catB2 和 tetA(60)，而 Bacillus、Rhizobium、Rossellomorea 和 Agrobacterium 是这些 ARGs 的宿主。这种富集是由于富集细菌种群中趋化基因 (如 cheW、cheC 和 cheD) 的上调和快速生物膜形成所致。值得注意的是，在 HDS 富集后，代表性的 ARGs，如 cat、macB 和 rpoB，在稻田土壤中分别显著减少了 36%、85.7%和 72%。我们的研究为铁载体作为一种捕获剂在环保条件下减少土壤环境中的 ARGs 及其载体提供了新的思路。
