Butt M M, Johnston H S, Scothorne R J
J Anat. 1985 Aug;141:231-9.
In order to perform morphometric analysis of the nasal (salt) glands of domestic duck it was necessary to standardise the regime of fixation and processing for this tissue. This paper reports the results of these experiments in which the nasal glands of nine groups of ducks were perfused by fixatives containing various concentrations of glutaraldehyde; each group included three secreting ducks and three non-secreting control ducks. Fixative osmolality ranged from 300-825 m-osmol. The resulting effects on the electron microscopical appearances of mitochondria and intercellular spaces were assessed. The fixative finally selected as most satisfactory for both control and secreting tissue contained 3.5% glutaraldehyde and 3% dextran in sodium cacodylate buffer, giving an osmolality range of 580-650 m-osmol.
为了对家鸭的鼻（盐）腺进行形态计量分析，有必要对该组织的固定和处理方法进行标准化。本文报告了这些实验的结果，其中九组鸭子的鼻腺用含有不同浓度戊二醛的固定剂进行灌注；每组包括三只分泌型鸭子和三只非分泌型对照鸭子。固定剂的渗透压范围为300 - 825毫渗摩尔。评估了其对线粒体和细胞间隙电子显微镜外观的影响。最终选择的对对照组织和分泌组织都最满意的固定剂是在二甲胂酸钠缓冲液中含有3.5%戊二醛和3%葡聚糖，渗透压范围为580 - 650毫渗摩尔。
