Reith A, Kraemer M, Vassy J
Scan Electron Microsc. 1984(Pt 2):645-51.
The influence on cellular and subcellular structure was studied by comparing perfusion versus immersion fixation with cacodylate buffered glutaraldehyde, osmium or glutaraldehyde immersion fixation with two vehicles, phosphate and cacodylate buffer. The study was performed in the same animal using the left liver lobe for the four immersion fixation experiments and the remaining lobes for perfusion. The preparative steps were done under identical conditions in one operation. The mode of fixation was the most influential factor with clear (15%) increases for the volume of hepatocytes (mainly their cytoplasm), and the mitochondria (30%), when comparing immersion to perfusion fixation. This mitochondrial enlargement was particularly displayed in the organelles' average profile area, being more than doubled (211%) in immersion fixation. Similarly high profile enlargements (150%) were also found after osmium immersion fixation. Changes in nuclei were minor compared to the cytoplasm. There was a good correlation between the volume changes of the hepatocyte cytoplasm and the mitochondria (r = 0.85, p less than 0.01). Qualitative results revealed that there exists a zone of good electron microscopic preservation 2-3 cell layers beneath the block edge in immersion fixed material, whereas perfusion fixed tissue displays a homogeneous fine structure.
通过比较用二甲胂酸盐缓冲戊二醛进行灌注固定与浸泡固定、用两种媒介（磷酸盐和二甲胂酸盐缓冲液）进行戊二醛浸泡固定与锇固定，研究了对细胞及亚细胞结构的影响。该研究在同一动物身上进行，四个浸泡固定实验使用左肝叶，其余肝叶用于灌注。制备步骤在一次操作中于相同条件下完成。固定方式是最具影响力的因素，与灌注固定相比，浸泡固定时肝细胞体积（主要是其细胞质）明显增加（15%），线粒体体积增加（30%）。这种线粒体增大尤其体现在细胞器的平均轮廓面积上，浸泡固定时该面积增加超过一倍（211%）。同样，锇浸泡固定后也发现轮廓有很高的增大比例（150%）。与细胞质相比，细胞核的变化较小。肝细胞细胞质与线粒体的体积变化之间存在良好的相关性（r = 0.85，p < 0.01）。定性结果显示，在浸泡固定材料中，在块边缘下方2 - 3个细胞层处存在一个电子显微镜保存良好的区域，而灌注固定组织呈现出均匀的精细结构。
