College of Biosystems Engineering and Food Science, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China.
College of Biosystems Engineering and Food Science, National Engineering Laboratory of Intelligent Food Technology and Equipment, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang University, Hangzhou 310058, China; Innovation Center of Yangtze River Delta, Zhejiang University, Jiaxing 314102, China.
Int J Biol Macromol. 2024 Oct;277(Pt 3):134242. doi: 10.1016/j.ijbiomac.2024.134242. Epub 2024 Jul 29.
In order to achieve high cell adhesion and growth efficiency on scaffolds for cultured meat, animal materials, especially gelatin, are necessary though the disadvantages of weak mechanical properties and poor stability of their hydrogel scaffolds are present during cell cultivation. Here, we use rice bran as a kind of filling and supporting materials to develop a composite scaffold with gelatin for fish cell cultivation, where rice bran is also inexpensive from high yield fibrous agricultural by-product. The rice bran (with a proportion of 1, 3, 5, 7, 10 to 3 of gelatin) could evenly distributed in the three-dimensional network composed of gelatin hydrogel. It contributed to delaying swelling and degradation rates, fixing water and improving elastic modulus. It is important that rice bran-gelatin hydrogel scaffolds (especially the hydrogel with 70 % rice bran, db) promoted piscine satellite cells (PSCs) proliferation effectively compared to the pure gelatin hydrogel, and the former could also support the differentiation of PSCs. Overall, this work showed a positive promotion to explore new source of scaffold materials like agricultural by-product for reducing the cost of cell cultured meat production.
为了实现在培养肉支架上的高细胞黏附和生长效率，动物材料，特别是明胶，是必要的，尽管它们的水凝胶支架的机械性能弱和稳定性差的缺点在细胞培养期间存在。在这里，我们使用米糠作为一种填充和支撑材料，为鱼类细胞培养开发一种具有明胶的复合支架，米糠也是一种由高产纤维农业副产品而来的廉价材料。米糠（与明胶的比例为 1、3、5、7、10 比 3）可以均匀分布在由明胶水凝胶组成的三维网络中。它有助于延缓溶胀和降解速率，固定水分并提高弹性模量。重要的是，米糠-明胶水凝胶支架（特别是含有 70%米糠的 db）与纯明胶水凝胶相比，能有效促进鱼类卫星细胞（PSCs）的增殖，而且前者还能支持 PSCs 的分化。总的来说，这项工作对探索农业副产品等新型支架材料来源以降低细胞培养肉生产成本起到了积极的推动作用。
