State Key Laboratory of Food Science and Resources, Nanchang University, China-Canada Joint Laboratory of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, Jiangxi, China.
J Agric Food Chem. 2024 Nov 6;72(44):24359-24373. doi: 10.1021/acs.jafc.3c09535. Epub 2024 Jul 31.
Intestinal stem cells (ISCs) are necessary to maintain intestinal renewal. Here, we found that the β-glucan (HBG) alleviated pathological symptoms and promoted the proliferation of intestinal stem cells in colitis mice. Notably, metabolomics studies showed that docosahexaenoic acid (DHA) was significantly increased by the HBG treatment. DHA is a ligand for peroxisome proliferator-activated receptor α (PPARα), which can promote ISC proliferation. Expectedly, HBG facilitated the expression of intestinal PPARα and the proliferation of ISCs in colitis mice. Further experiments verified that DHA significantly facilitated the expression of PPARα and the proliferation of ISCs in intestinal organoids. Intriguingly, the effect of DHA on ISC proliferation was reversed by the PPARα inhibitor. Together, our data indicate that HBG might accelerate PPARα-mediated ISC proliferation through DHA. This provides new insights into the effective application of polysaccharides in maintaining intestinal homeostasis.
肠干细胞（ISCs）是维持肠道更新所必需的。在这里，我们发现 β-葡聚糖（HBG）可缓解结肠炎小鼠的病理症状并促进肠干细胞的增殖。值得注意的是，代谢组学研究表明 HBG 处理可显著增加二十二碳六烯酸（DHA）。DHA 是过氧化物酶体增殖物激活受体 α（PPARα）的配体，可促进 ISC 增殖。正如预期的那样，HBG 促进了结肠炎小鼠中肠道 PPARα 的表达和 ISC 的增殖。进一步的实验验证了 DHA 可显著促进肠类器官中 PPARα 的表达和 ISC 的增殖。有趣的是，PPARα 抑制剂可逆转 DHA 对 ISC 增殖的作用。总之，我们的数据表明 HBG 可能通过 DHA 加速 PPARα 介导的 ISC 增殖。这为多糖在维持肠道内稳态中的有效应用提供了新的见解。
