Key Laboratory of Geriatric Nutrition and Health, Beijing Technology and Business University, Ministry of Education, 100048, People's Republic of China; Key Laboratory of Brewing Molecular Engineering of China Light Industry, Beijing Technology and Business University, Beijing 100048, PR China.
Int J Biol Macromol. 2024 Aug;275(Pt 1):133993. doi: 10.1016/j.ijbiomac.2024.133993. Epub 2024 Jul 31.
Jiuzao is the main solid by-products of Baijiu industry, which contain a high amount of underutilized cellulose and proteins. In recent years, cellulose nanofibers mixed with proteins to prepare biodegradable bio-based film materials have received widespread attention. In this study, we propose a novel method to simultaneously extract kafirin and cellulose from strong-flavor type of Jiuzao, and modify cellulose to prepare cellulose nanofibers by the TEMPO (2,2,6,6-tetramethylpiperidine-1-oxide) oxidation-pressure homogenization technique, and finally mix kafirin with cellulose nanofibers to prepare a new biodegradable bio-based composite film. Based on the analysis of one-way and response surface experiments, the highest purity of cellulose was 82.04 %. During cellulose oxidation, when NaClO was added at 25 mmol/g, cellulose nanofibers have a particle size of 80-120 nm, a crystallinity of 65.8°. Finally, kafirin and cellulose nanofibers were mixed to prepare films. The results showed that when cellulose nanofibers were added at 1 %, the film surface was smooth, the light transmittance was 60.8 %, and the tensile strength was 9.17 MPa at maximum, which was 104 % higher than pure protein film. The contact angle was 34.3°. This paper provides new ideas and theoretical basis for preparing biodegradable bio-based composite film materials, and improves the added value of Jiuzao.
酒糟是白酒工业的主要固体副产物，含有大量未充分利用的纤维素和蛋白质。近年来，将纤维素纳米纤维与蛋白质混合制备可生物降解的生物基薄膜材料受到了广泛关注。在本研究中，我们提出了一种从浓香型酒糟中同时提取麦醇溶蛋白和纤维素的新方法，并通过 TEMPO（2,2,6,6-四甲基哌啶-1-氧化物）氧化-压力均质技术对纤维素进行改性，制备纤维素纳米纤维，最后将麦醇溶蛋白与纤维素纳米纤维混合，制备新型可生物降解的生物基复合膜。通过单因素和响应面实验分析，纤维素的最高纯度为 82.04%。在纤维素氧化过程中，当添加 25mmol/g 的 NaClO 时，纤维素纳米纤维的粒径为 80-120nm，结晶度为 65.8%。最后，将麦醇溶蛋白和纤维素纳米纤维混合制备成膜。结果表明，当纤维素纳米纤维添加量为 1%时，膜表面光滑，透光率为 60.8%，拉伸强度达到 9.17MPa，比纯蛋白膜提高了 104%。接触角为 34.3°。本文为制备可生物降解的生物基复合膜材料提供了新的思路和理论依据，提高了酒糟的附加值。
