Han Wenyan, Mu Yongping, Zhang Zhihui, Su Xiulan
Clinical Medical Research Center, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010050, P.R. China.
Department of Clinical Laboratory, The Affiliated People's Hospital of Inner Mongolia Medical University, Hohhot, Inner Mongolia 010020, P.R. China.
Oncol Lett. 2018 Aug;16(2):2416-2426. doi: 10.3892/ol.2018.8934. Epub 2018 Jun 8.
microRNA-30c (miR-30c) is a member of the miR-30s family, which is known to serve important roles in the occurrence and development of numerous tumor types. Our previous microarray analysis of extracted RNA from tissue samples was conducted to examine the expression of miR-30c and predict miR-30c target genes. In the present study, it was determined that the expression of miR-30c was differentially expressed in 82 paired gastric cancer (GC) and paracancerous tissues. Cellular expression of miR-30c in two GC cell lines MKN-45, MKN-74 and one non-cancer cell line GES-1 was modified using the miR-30c-mimic and miR-30c-inhibitor reagents, in a series of transfection experiments. Following transfection of cancer and non-cancer cell lines with the miR-30c-mimic, cell proliferation and apoptosis rates were increased. Compared with the NC group, MKN-74 cell proliferation was significantly inhibited (P<0.05) following transfection with the miR-30c-mimic at 48 and 24 h, GES-1 was significantly inhibited (P<0.05) at 24 and 48 h, and apoptosis was significantly reduced in transfected MKN-74 cells (P<0.05). The clinicopathological data and the expression of BCL-9 and miR-30c in patients with GC were used to identify associations. The expression levels of miR-30c were associated with age. Western blot analysis demonstrated that the BCL-9 expression levels in MKN-74 cells were higher following transfection with the miR-30c-mimic, and were lower following transfection with the miR-30c-inhibitor, both compared with the negative control group. It was concluded that compared with the negative control group, the expression of miR-30c was low in GC tissues and may be involved in GC development via regulation of proliferation, apoptosis and the cell cycle.
微小RNA-30c（miR-30c）是miR-30家族的成员，已知其在多种肿瘤类型的发生和发展中发挥重要作用。我们之前对从组织样本中提取的RNA进行了微阵列分析，以检测miR-30c的表达并预测miR-30c的靶基因。在本研究中，确定miR-30c在82对胃癌（GC）组织和癌旁组织中差异表达。在一系列转染实验中，使用miR-30c模拟物和miR-30c抑制剂试剂改变了miR-30c在两种GC细胞系MKN-45、MKN-74和一种非癌细胞系GES-1中的细胞表达。用miR-30c模拟物转染癌细胞系和非癌细胞系后，细胞增殖和凋亡率增加。与阴性对照组相比，在转染miR-30c模拟物48小时和24小时后，MKN-74细胞增殖受到显著抑制（P<0.05），在24小时和48小时时GES-1细胞增殖受到显著抑制（P<0.05），并且转染后的MKN-74细胞凋亡显著减少（P<0.05）。利用GC患者的临床病理数据以及BCL-9和miR-30c的表达来确定相关性。miR-30c的表达水平与年龄相关。蛋白质印迹分析表明，与阴性对照组相比，转染miR-30c模拟物后MKN-74细胞中BCL-9的表达水平较高，而转染miR-30c抑制剂后BCL-9的表达水平较低。结论是，与阴性对照组相比，miR-30c在GC组织中的表达较低，并且可能通过调节增殖、凋亡和细胞周期参与GC的发生发展。
