黑色素瘤中的蛋白水解酶和纤溶酶原激活剂。
Proteolytic enzymes and plasminogen activator in melanoma.
作者信息
Fräki J E, Nieminen S, Hopsu-Havu V K
出版信息
J Cutan Pathol. 1979 Jun;6(3):195-200. doi: 10.1111/j.1600-0560.1979.tb01123.x.
Proteolytic activities were measured in extracts of human skin melanoma, lymphatic metastasis and in nonmalignant naevi by using various proteinase substrates as well as plasminogen activator assay. pH-optima for hydrolysis of various proteinase substrates by these tumors were found to be essentially the same as in healthy human skin. Melanoma extracts were found to especially readily hydrolyze N-alpha-benzoyl-DL-arginine beta-naphthylamine (BANA) at pH 5.8 in the presence of 1 mmol/l dithiothreitol and EDTA (cathepsin B1-like enzyme) as well as histones and p-tosyl-L-arginine methyl ester (TAME) at pH 7.5, and showed increased capacity to activate plasminogen when compared to nonmalignant naevus. The possible role of proteinases in malignant melanoma is discussed.
通过使用各种蛋白酶底物以及纤溶酶原激活剂测定法，对人皮肤黑色素瘤、淋巴转移灶和非恶性痣的提取物中的蛋白水解活性进行了测量。发现这些肿瘤对各种蛋白酶底物进行水解的最适pH值与健康人皮肤中的基本相同。在1 mmol/l二硫苏糖醇和乙二胺四乙酸存在的情况下，黑色素瘤提取物在pH 5.8时特别容易水解N-α-苯甲酰-DL-精氨酸β-萘胺（BANA）（组织蛋白酶B1样酶），在pH 7.5时容易水解组蛋白和对甲苯磺酰-L-精氨酸甲酯（TAME），并且与非恶性痣相比，其激活纤溶酶原的能力有所增强。文中讨论了蛋白酶在恶性黑色素瘤中的可能作用。
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