黑色素瘤中的蛋白水解酶和纤溶酶原激活剂。

Proteolytic enzymes and plasminogen activator in melanoma.

作者信息

Fräki J E, Nieminen S, Hopsu-Havu V K

出版信息

J Cutan Pathol. 1979 Jun;6(3):195-200. doi: 10.1111/j.1600-0560.1979.tb01123.x.

DOI:10.1111/j.1600-0560.1979.tb01123.x

Abstract

Proteolytic activities were measured in extracts of human skin melanoma, lymphatic metastasis and in nonmalignant naevi by using various proteinase substrates as well as plasminogen activator assay. pH-optima for hydrolysis of various proteinase substrates by these tumors were found to be essentially the same as in healthy human skin. Melanoma extracts were found to especially readily hydrolyze N-alpha-benzoyl-DL-arginine beta-naphthylamine (BANA) at pH 5.8 in the presence of 1 mmol/l dithiothreitol and EDTA (cathepsin B1-like enzyme) as well as histones and p-tosyl-L-arginine methyl ester (TAME) at pH 7.5, and showed increased capacity to activate plasminogen when compared to nonmalignant naevus. The possible role of proteinases in malignant melanoma is discussed.

摘要

通过使用各种蛋白酶底物以及纤溶酶原激活剂测定法，对人皮肤黑色素瘤、淋巴转移灶和非恶性痣的提取物中的蛋白水解活性进行了测量。发现这些肿瘤对各种蛋白酶底物进行水解的最适pH值与健康人皮肤中的基本相同。在1 mmol/l二硫苏糖醇和乙二胺四乙酸存在的情况下，黑色素瘤提取物在pH 5.8时特别容易水解N-α-苯甲酰-DL-精氨酸β-萘胺（BANA）（组织蛋白酶B1样酶），在pH 7.5时容易水解组蛋白和对甲苯磺酰-L-精氨酸甲酯（TAME），并且与非恶性痣相比，其激活纤溶酶原的能力有所增强。文中讨论了蛋白酶在恶性黑色素瘤中的可能作用。